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  • 平臺(tái)編號(hào):bio-50153
  • 國(guó)際編號(hào):ATCC VR-3138
  • 拉丁屬名: Vaccinia virus ts mutant
  • 規(guī)格:frozen
  • 用途:ATCC原裝進(jìn)口
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ATCC? Number:VR-3138?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts44
Depositors: S. Dales and R. Condit
Biosafety Level:2 [BSL 2 Vaccination reommended for laboratory staff manipulating vaccinia, mondeypox, or cowpox viruses [16172602] ]
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing
Store at : -70 C or lower
Comments : Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category B, indicating that it induces foci of a fibrous viroplasmic matrix substance in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to Vaccinia map location J4 [70405]
Related Products: VR-1441 Vaccinia virus, strain IHD-W

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