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ATCC? Number:VR-3130?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant deposited as Vaccinia virus, Orthopoxvirus
Strain: IHD-W Dts4 (Dales isolate 260)
Original Source: derived from existing strain (This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCCVR-1441) which in turn was derived from the IHD-J strain.)
Depositors: S Dales, RC Condit, S Dales
Biosafety Level:2 [CDC BMBL recommends vaccination. [61305] ]
Shipped: frozen
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Tested Positive for Mycoplasma : No
Incubation : Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection [not 100% confluent].
Effect : Yes, Cytopathic effect: Rounding and sloughing of infected cells
Comments : Selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5oC. This mutant was not assigned to a Dales EM category. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U4.
References : 33180: Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 61241: Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 61305: . Biosafety in Microbiological and Biomedical Laboratories 4th ed.. : ; 1999. 70405: Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570