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  • 平臺編號:bio-50159
  • 國際編號:ATCC VR-3118
  • 拉丁屬名: Vaccinia virus ts mutant
  • 規(guī)格:frozen
  • 用途:ATCC原裝進口
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  • 注意事項:僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準)

ATCC? Number:VR-3118?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts42
Depositors: S. Dales, R. Condit
Biosafety Level:2 [BSL 2 Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox and cowpox viruses ]
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing
Store at : -70 C or colder
Comments : This conditional lethal mutant, originally designated plaque isolate no. 6626, was isolated from the IHD-W strain of Vaccinia by S. Dales by mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM categories D, inducing foci of viroplasma and DNA paracrystals in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location G2, a viral positive transcription elongation factor. In the absence of G2 protein, transcripts are shorter than in the presence of G2 protein.Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox and cowpox viruses
Related Products: VR-1441 Vaccinia virus, strain IHD-W

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