ATCC? Number:VR-3118?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts42
Depositors: S. Dales, R. Condit
Biosafety Level:2 [BSL 2 Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox and cowpox viruses ]
Shipped: frozen
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Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing
Store at : -70 C or colder
Comments : This conditional lethal mutant, originally designated plaque isolate no. 6626, was isolated from the IHD-W strain of Vaccinia by S. Dales by mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM categories D, inducing foci of viroplasma and DNA paracrystals in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location G2, a viral positive transcription elongation factor. In the absence of G2 protein, transcripts are shorter than in the presence of G2 protein.Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox and cowpox viruses
Related Products: VR-1441 Vaccinia virus, strain IHD-W