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平臺(tái)編號(hào)：bio-50161
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國(guó)際編號(hào)：ATCC VR-3127
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拉丁屬名： Vaccinia virus ts mutant
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
中文名稱暫無
ATCC? Number:VR-3127?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts38
Depositors: S Dales, R. Condit
Biosafety Level:2 [BSL 2 ]
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 55 CO2
Effect : Cytopathic effects in culture include enlargement and rounding of cells, followed by cell death and sloughing
Store at : -70 C or colder
Comments : This conditional lethal mutant, originally designated plaque isolate no. 6389, was gnerated from the IHD-W strain of Vaccinia by S. Dales by mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. It was assigned to Dales EM category C, indicating that it induces rudimentary virus membranes with spicules in foci of viroplasm in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D5, an ATPase required for DNA replication. Shifting infection conditions from permissive to non-permissive temperature results in an abrupt cessation of DNA synthesis, described as a fast stop phenotype.Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses [70405]
Related Products: VR-1441 Vaccinia virus, IHD-W
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts38
Depositors: S Dales, R. Condit
Biosafety Level:2 [BSL 2 ]
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 55 CO2
Effect : Cytopathic effects in culture include enlargement and rounding of cells, followed by cell death and sloughing
Store at : -70 C or colder
Comments : This conditional lethal mutant, originally designated plaque isolate no. 6389, was gnerated from the IHD-W strain of Vaccinia by S. Dales by mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. It was assigned to Dales EM category C, indicating that it induces rudimentary virus membranes with spicules in foci of viroplasm in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D5, an ATPase required for DNA replication. Shifting infection conditions from permissive to non-permissive temperature results in an abrupt cessation of DNA synthesis, described as a fast stop phenotype.Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses [70405]
Related Products: VR-1441 Vaccinia virus, IHD-W
