ATCC? Number:VR-3124?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant deposited as Vaccinia virus, Orthopoxvirus
Strain: IHD-W Dts23
Depositors: S Dales, R Condit
Biosafety Level:2 [BSL 2 Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses [16172602] ]
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects in cell culture include cell rounding and death, with eventual sloughing
Store at : -70 C or lower
Comments : Not described in the original publication, but persumed to have been selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5 C. This mutant was not assigned to a Dales EM category. The mutation was assigned by complementation analysis to vaccinia map location E(2-8) (exact location not known). [70405]
Related Products: VR-1441 (Vaccinia virus, strain: IHD-W)