ATCC? Number:VR-3133?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts19
Depositors: S Dales, R. Condit
Biosafety Level:2 [BSL 2 ]
Shipped: frozen
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Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : Duration: 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing
Store at : -70 C or colder
Comments : This conditional lethal mutant, originally designated plaque isolate no. 2096, was isolated from the IHD-W strain of Vaccinia by S. Dale after mutagenesis with nitrosoguanidine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category B, indicating that it induces foci of a fibrous viroplasmic matrix substance without membranes in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location E11, a 129 amino acid core protein that is synthesized late during infection.Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses [70405]
Related Products: VR-1414 Vaccinia virus, strain IHD-W