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  • 平臺(tái)編號(hào):bio-50166
  • 國(guó)際編號(hào):ATCC VR-3133
  • 拉丁屬名: Vaccinia virus ts mutant
  • 規(guī)格:frozen
  • 用途:ATCC原裝進(jìn)口
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  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫(kù)為準(zhǔn))

ATCC? Number:VR-3133?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts19
Depositors: S Dales, R. Condit
Biosafety Level:2 [BSL 2 ]
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : Duration: 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing
Store at : -70 C or colder
Comments : This conditional lethal mutant, originally designated plaque isolate no. 2096, was isolated from the IHD-W strain of Vaccinia by S. Dale after mutagenesis with nitrosoguanidine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category B, indicating that it induces foci of a fibrous viroplasmic matrix substance without membranes in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location E11, a 129 amino acid core protein that is synthesized late during infection.Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses [70405]
Related Products: VR-1414 Vaccinia virus, strain IHD-W

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