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  • 平臺編號:bio-50173
  • 國際編號:ATCC VR-3132
  • 拉丁屬名: Vaccinia virus ts mutant
  • 規(guī)格:frozen
  • 用途:ATCC原裝進(jìn)口
  • 服務(wù)費(fèi)用:
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  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

ATCC? Number:VR-3132?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts14
Depositors: S Dales, R. Condit
Biosafety Level:2 [BSL 2 Vaccination is recommended for laboratory staff working with vaccinia, monkeypox and cowpox viruses [16172602] ]
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects in culture including enlargement and rounding of cells, followed by death and sloughing
Store at : -70 C or colder
Comments : Selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM categories B and P, characterized by induction of viroplasm in infected cells, and generation of immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location A24, the 132-kDa RNA polymerase subunit. [70405]
Related Products: VR-1441 Vaccinia virus, strain IHD-W

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