ATCC? Number:VR-3132?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts14
Depositors: S Dales, R. Condit
Biosafety Level:2 [BSL 2 Vaccination is recommended for laboratory staff working with vaccinia, monkeypox and cowpox viruses [16172602] ]
Shipped: frozen
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Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects in culture including enlargement and rounding of cells, followed by death and sloughing
Store at : -70 C or colder
Comments : Selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM categories B and P, characterized by induction of viroplasm in infected cells, and generation of immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location A24, the 132-kDa RNA polymerase subunit. [70405]
Related Products: VR-1441 Vaccinia virus, strain IHD-W