ATCC? Number:VR-1441?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus deposited as Vaccinia virus, Orthopoxvirus
Strain: IHD-W
Original Source: The IHD-W subtype was derived in Japan from IHD-J strain ofvaccinia by selection for the ability to induce distinct clear plaques in culture.
Depositors: S Dales
Biosafety Level:2
Shipped: frozen
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Host Organism : L2 cells (ATCCCCL-149); chicken embryo fibroblasts most mammalian cells and tissues
Incubation : Duration: 1-5 days at 37C
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture
Store at : -70C
Comments : IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s. IHD-W differs from the IHD-J parent strain, in that IHD-W is unable to induce an active hemagglutinin in the plasma membrane and does elicit polykaryoctosis. The host range includes most mammalian cells and tissues. Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4). IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s. IHD-W differs from IHD-J in that IHD-W is HA(-) and IHD-J is HA(+). -70C