ATCC? Number:VR-3112?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts12
Depositors: S. Dales, R. Condit
Biosafety Level:2 [BSL 2 Vaccination recommended for laboratory personnel manipulating vaccinia, monkeypox, or cowpox viruses [16172602] ]
Shipped: frozen
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Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects in cell culture include enlargement and rounding of cells, followed by death and slouging
Store at : -70 C or colder
Comments : Not described in the original publication, but presumed to have been selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5 C. This mutant was not assigned to a Dales EM category. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D5, a viral ATPase required for DNA replication. [70405]
Related Products: VR-1441 Vaccinia virus, strain IHD-W