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ATCC? Number:VR-156?
Agent: Vaccinia virus deposited as Vaccinia virus
Strain: IHD
Original Source: derived from existing strain (This material was derived from the New York City Department of Health strain. The history as given by the original contributor, Dr. J. Earle Officer, United States Army Biological Laboratories, Fort Detrick, Frederick, Maryland was: Strain originally derived from Board of Health strain (BH) of New York City Board of Health. Obtained by Dr. R. F. Parker from Dr. J. E. Smadel. Many IC mouse passages made (# unknown). Received by Dr. R. L. Thompson, 1939, as infected mouse brain. Passaged approx. 50 times IC in mice. Received by Dr. F. Shabel 2 Jul 1952 as infected mouse brain. Passage 1 mouse IC and 1 egg CAM. Received by Fort Detrick 15 Nov l954. 4 egg CAM.)
Depositors: JE Officer
Biosafety Level:2 [CDC BMBL recommends vaccination. (Vaccination recommended for laboratory staff manipulating vaccinia, monkeypox, or cowpox viruses [CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4].)]
Shipped: frozen
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Host Organism : Mus musculusOryctolagus cuniculusHomo sapiensGallus gallus
Incubation : Temperature: 37.0°C Duration: 4 to 6 days
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture Yes, in vivo effects: causes death of mice
Store at : -70°C (or colder)
Comments : The virus will agglutinate chicken RBC.
References : 32715: Parker RF, et al. Further studies of the infectious unit of Vaccinia. J. Exp. Med. 74: 263-281, 1941.