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平臺編號：bio-50206
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國際編號：ATCC VR-2104
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拉丁屬名： Hu/Australia/10-25-10/77/L
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Hu/Australia/10-25-10/77/L
ATCC? Number:VR-2104?
Classification: Reoviridae, Rotavirus
Agent: Human rotavirus
Strain: Hu/Australia/10-25-10/77/L
Original Source: Stools of human neonate in a Melbourne maternity hospital in 1977
Depositors: G Barnes
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : MA-104 cells (ATCCCRL-2378); FRhL2 cells MA-104 cells (ATCCCCL-2378); FRhL2
Incubation : Temperature: 37.0°C
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture
Comments : Patent Deposit. The virus shows typical rotavirus morphology by electron microscopy. It has a diameter of 65-75 nm for the double-shelled form and 55-65 nm for the single-shelled form. Following serial passage in TC this virus appears to be attenuated. Cultivated in MA104 cells--see Sato et al. (Archives of Virology 1981, 69: 155-160). Pretreat inocula with 20 ug/ml trypsin, adsorb to cells and add medium with 0.5 ug/ml trypsin. See also Dyall-Smith (Nucleic Acids Research 1984, 12:3073). The virus shows typical rotavirus morphology by electron microscopy. It has a diameter of 65-75nm for the double-shelled form and 55-65nm for the single shelled form. Following serial passage in TC this virus appears to be attenuated. Tissue culture supernatant Dulbecco's medium + 1 ug/ml porcine pancreas type IX trypsin Dr. Graeme Barnes, Royal Children's Hospital
References : 33275: Albert MJ, et al. Live attenuated human rotavirus vaccine. US Patent 4,624,850 dated Nov 25 1986
Classification: Reoviridae, Rotavirus
Agent: Human rotavirus
Strain: Hu/Australia/10-25-10/77/L
Original Source: Stools of human neonate in a Melbourne maternity hospital in 1977
Depositors: G Barnes
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : MA-104 cells (ATCCCRL-2378); FRhL2 cells MA-104 cells (ATCCCCL-2378); FRhL2
Incubation : Temperature: 37.0°C
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture
Comments : Patent Deposit. The virus shows typical rotavirus morphology by electron microscopy. It has a diameter of 65-75 nm for the double-shelled form and 55-65 nm for the single-shelled form. Following serial passage in TC this virus appears to be attenuated. Cultivated in MA104 cells--see Sato et al. (Archives of Virology 1981, 69: 155-160). Pretreat inocula with 20 ug/ml trypsin, adsorb to cells and add medium with 0.5 ug/ml trypsin. See also Dyall-Smith (Nucleic Acids Research 1984, 12:3073). The virus shows typical rotavirus morphology by electron microscopy. It has a diameter of 65-75nm for the double-shelled form and 55-65nm for the single shelled form. Following serial passage in TC this virus appears to be attenuated. Tissue culture supernatant Dulbecco's medium + 1 ug/ml porcine pancreas type IX trypsin Dr. Graeme Barnes, Royal Children's Hospital
References : 33275: Albert MJ, et al. Live attenuated human rotavirus vaccine. US Patent 4,624,850 dated Nov 25 1986
