ATCC? Number:VR-624?
Classification: Herpesviridae, Mardivirus
Agent: Gallid herpesvirus 2 deposited as Marek's disease virus
Strain: GA5
Original Source: chicken tissue with Marek's disease
Depositors: BW Calnek, KA Schat
Biosafety Level:2
Shipped: frozen
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Host Organism : chicken kidney cells; chicken chicken kidney cells in tissue culture; chicken
Incubation : Duration: 5-7 days at 38.5C in TC
Effect : Yes, in vitro effects: Cytopathic effects (focal lesions) chicken kidney cells in tissue culture Yes, in vivo effects: Marek's disease lesion in chickens
Comments : Early lots prepared from feather follicle of pathogen free chickens (Avian Dis. 14:219-233; Appl. Microbiol. 20:723- 726). For TC titration, adsorb 0.1ml inoculum to CEK monolayer, feed and overlay with agar 24hr PI. Read day 7 PI.
References : 3471: Calnek BW, Hitchner SB. Highly potent, viable and stable cell-free virus preparations from cells infected with cell-associated viruses and method for obtaining the same. US Patent 3,783,098 dated Jan 1 1974 33720: Eidson CS, Schmittle SC. Studies on acute Marek's disease. I. Characteristics of isolate GA in chickens. Avian Dis. 12: 467-476, 1968. PubMed: 4301071