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ATCC? Number:VR-604?
Classification: Reoviridae, Reovirus
Agent: Bluegill virus
Strain: EFDL Clone B
Original Source: Dermal scrapings from bluegill (fish) (Lepomis macrochirus)
Depositors: KE Wolf, MC Quimby
Biosafety Level:2
Shipped: frozen
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Host Organism : bluegill fry (BF-2) cells (ATCCCCL-91) BF-2 cells (ATCCCCL-91); RTG-2 cells (ATCCCCL-55)
Incubation : Temperature: 25.0°C Duration: 3 days
Effect : Yes, in vitro effects: elongate pyknosis followed by lysis
Comments : The only cells thus far known to replicate this agent are those from fishes, but there is an additional restriction: cells with a fibroblast-like morphology are required. The agent is not replicated by fathead minnow cells (ATCCCCL 42). Previously believed to be a paramyxovirus. The only cells thus far known to replicate this agent are those from fishes, but there is an additional restriction: cells with a fibroblast-like morphology are required. The agent is not replicated by fathead minnow cells (ATCCCCL 42).
References : 33699: Hoffman GL, et al. Epitheliocystis, a new infectious disease of the bluegill (Lepomis macrochirus). Antonie van Leeuwenhoek 35: 146-158, 1969. PubMed: 4987372 33700: Wolf K, Quimby MC. Fish viruses: buffers and methods for plaquing eight agents under normal atmosphere. Appl. Microbiol. 25: 659-664, 1973. PubMed: 4349252