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ATCC® Number:VR-953™
Classification: Parvoviridae, Parvovirus, Feline panleukopenia virus
Agent: Canine parvovirus
Strain: Cornell-780916, attenuated
Original Source: Feces of dog with hemorrhagic enteritis
Depositors: LE Carmichael
Biosafety Level:2
Shipped: frozen
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Host Organism : primary and secondary dog kidney cells Mv 1 Lu cells (ATCCCCL-64); CRFK cells (ATCCCCL-94); A-72 cells (ATCCCRL-1542)
Incubation : Max Temperature: 37.0°C Min Temperature: 35.0°C Duration: 3-4 days
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture
Comments : Antigenically indistinguishable from feline panleukopenia virus and mink enteritis virus, but it has a broader host range and different HA spectrum, especially as regards pH conditions. Non-pathogenic for neonatal pups. Hemagglutinates swine, Rh+ monkey & cat RBC at pH 6.0-7.5. On passage, add virus 2 hrs after setting host cells. After 8-10 passage in feline cells may fail to immunize dogs.
References : 33222: Appel MJ, et al. Isolation and immunisation studies of a canine parco-like virus from dogs with haemorrhagic enteritis. Vet. Rec. 105: 156-159, 1979. PubMed: 516347 34040: Carmichael LE, et al. A modified live canine parvovirus strain with novel plaque characteristics. I. Viral attenuation and dog response. Cornell Vet. 71: 408-427, 1981. PubMed: 7318443