ATCC? Number:VR-714?
Classification: Rhabdovirus, Novirhabdovirus, fish virus
Agent: Infectious hematopoietic necrosis virus
Strain: Chinook salmon
Original Source: Homogenate of whole fry, Chinook salmon (Oncorhynchus tshawytscha)
Depositors: KE Wolf, MC Quimby
Biosafety Level:3
Shipped: freeze-dried
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Host Organism : rainbow trout gonad (RTG-2) cells (ATCCCCL-55) RTG-2 cells (ATCCCCL-55); FHM cells (ATCCCCL-42) at pH 7.6 to 8.0
Incubation : Temperature: 15.0°C Duration: 3-4 days
Effect : Yes, in vitro effects: Perforated plaques with central necrosis and contracted edges. Cells at edges have marginated chromatin
Comments : Temperature sensitive (56C for 15 minutes), 170 X 70 nm in size. Ether-sensitive, acid and glycerol-labile. Does not hemagglutinate, replicates poorly if at all at 20C.
References : 33839: Amend DF. . Trans. Am. Fish. Soc. 98: 796, 1969. 33840: Amend DF, Chambers VA. . J. Fish. Res. Board Can. 27: 1285, 1970. 33841: Darlington RW, et al. Fish rhabdoviruses: morphology and ultrastructure of North American salmonid isolates. Arch. Gesamte Virusforsch. 39: 257-264, 1972. PubMed: 4117916