ATCC? Number:VR-2332?
Classification: Arteriviridae
Agent: Porcine respiratory and reproductive syndrome virus
Strain: BIAH-001
Original Source: Combined tissues obtained from a SIRS infected piglet
Depositors: Boehringer Ingelheim Corp.
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : MA-104 cells (ATCCCRL-2378) MA-104 cells (ATCCCCL-2378); other simian cell lines
Incubation : Temperature: 35.0°C Duration: 2-5 days in stationary closed system without CO2
Effect : Yes, in vitro effects: Cytopathic effects begins with isolated cells 48-72 hours post infection. Cells become swollen then detach. CPE progresses rapidly once it starts
Comments : Virus may cause disease in swine, specifically respiratory disease and stillbirth in pregnant sows. The virus is fastidious, non-hemagglutinating enveloped RNA virus. It is heat labile and grows best at 34-35C. Inoculate 80-90% confluent monolayer of cell. Replace media 24 hours post infection.
References : 24513: Chaladek DW, et al. Method of growing and attenuating a viral agent associated with mystery swine disease. US Patent 5,476,778 dated Dec 19 1995