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ATCC? Number:VR-995?
Classification: Picornavirus, Cardiovirus, Theilovirus
Agent: Theiler's murine encephalomyelitis virus deposited as Mouse encephalomyelitis
Strain: BeAn 8386
Original Source: Feral mouse, Rockefeller arbovirus unit, Belem, Brazil, 1957
Depositors: H. L. Lipton
Biosafety Level:2
Shipped: frozen
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Host Organism : Mus musculusBHK-21(C-13) cells (ATCCCCL-10) Animal hosts: mouse, hamster
Incubation : Production host: BHK-21 (C13) cells (ATCCCCL-10) Plant cells to be infected one day in advance. Infect at 80-90% confluence using a MOI of about 0.1. Refeed with EMEM containing 2% FBS, and incubate at 37℃, 5% CO2, for 1-2 days.
Effect : Cell rounding and sloughing in cell culture; demyelinating disease in mice in vivo.
Store at : Store at -70℃ or colder
Comments : Cross-neutralizes with other TMEV fully but does not cross-neutralize with the other cardioviruses (Mengo EMCV, etc.).