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ATCC? Number:VR-1735?
Classification: Orthomyxoviridae, Influenzavirus B
Agent: Influenza B virus
Strain: B/Taiwan/2/62 (TC-adapted)
Original Source: Patient in Taiwan, 1962.
Depositors: A.P. Kendal and ATCC
Biosafety Level:2
Shipped: frozen
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Host Organism : Production Host: MDCK (ATCCCCL-34)
Incubation : For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33.0°C in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. In TC, ATCCVR-1735 should be grown in serum free media that contains 1.0 μg/mL TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock).
Effect : CPE (cell rounding, degeneration, sloughing)
Store at : -70.0°C or colder
Comments : Derived by adaptation of egg-passage B/Taiwan/2/62 (ATCCVR-295) to MDCK (ATCCCCL-34) cells at ATCC. (Note: ATCCVR-295 and ATCCVR-1735 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture).