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平臺(tái)編號(hào)：bio-50864
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國際編號(hào)：ATCC VR-347
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拉丁屬名： Chlamydia trachomatis Serovar Ba
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
沙眼衣原體血清型BA
Chlamydia trachomatis Serovar Ba 拉丁名
(ATCC? VR-347?) 統(tǒng)一編號(hào)
Classification Chlamydiaceae, Chlamydia Deposited As Trachoma serotype ba Agent Chlamydia trachomatis Serovar Ba Strain Apache-2
Applications 用途 Sexually Transmitted Disease Research
Biosafety Level 生物安全等級(jí) 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen 1 mL per vial
Storage Conditions 保藏方法 -70°C or colder
Chlamydia trachomatis ATCC VR-347
Comments 注釋 FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
Please see ATCC Virology Guide for more detailed information on propagation.
Effect on Host Intracellular inclusion bodies visualized by fluorescent staining
Recommended Host McCoy cells (ATCC CRL-1696)
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 36°C
Recommendations for Infection: For best results, infection should be performed on a 80-100% confluent, 24-48 hour old cellular monolayer.
Sonicate seed material for 20 seconds at approximately 240W to disrupt cells.
Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2).
Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour. End adsorption by adding agent growth medium.
Incubation: Incubate infected culture for 72 hours at 36°C in a humidified 5% CO2 atmosphere.
Preservation: Controlled rate freeze.
Effect on Host Intracellular inclusion bodies visualized by fluorescent staining
Name of Depositor 寄存人 E Jawetz
Special Collection ATCC Source
Eye of an Apache Indian child from San Carlos, Arizona
References 參考文獻(xiàn) Hanna L, et al. Trachoma viruses isolated in United States. I. Growth in embryonated eggs. Proc. Soc. Exp. Biol. Med. 104: 142-145, 1960. PubMed: 14399529
