ATCC? Number:VR-1516?
Classification: Adenoviridae, Mastadenovirus, Human adenovirus C
Agent: Human adenovirus 5
Strain: Adenovirus Type 5 Reference Material
Depositors: Adenovirus Reference Material Working Group (ARMWG)
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tested Positive for Mycoplasma : No
Incubation : Temperature: 37.0°C
Store at : -80°C (Vials have not been tested sufficiently at liquid nitrogen (vapor or liquid) temperatures. At this time it is not certain whether or not the seal will be maintained at cryogenic temperatures.)
References : 61226: Hutchins B Development of a reference material for characterizing adenovirus vectors Bioprocess. J. 125-28, 2002 Available from: BioProcessing Journal 61227: Hutchins B, et al. Working toward an adenoviral vector testing standard. Mol. Ther. 2: 532-534, 2000. PubMed: 11124052 61229: Nyberg-Hoffman C, et al. Sensitivity and reproducibility in adenoviral infectious titer determination.. Nat. Med. 3: 808-811, 1997. PubMed: 9212113 61230: Smith GJ III, et al. Fast and accurate method for quantitating E. coli host-cell DNA contamination in plasmid DNA preparations.. BioTechniques 26: 518-526, 1999. PubMed: 10090994 61231: Hoffman K. Strategies for host cell protein analysis. BioTechniques 13: 38-45, 2000. 61232: Vallekamp G, et al. Empty capsids in column-purified recombinant adenovirus preparations.. Hum. Gene Ther. 12: 1923-1936, 2001. PubMed: 11589834 61233: Huyghe BG, et al. Purification of a type 5 recombinant adenovirus encoding human p53 by column chromatography.. Hum. Gene Ther. 6: 1403-1416, 1995. PubMed: 8573613 61234: Shabram PW, et al. Analytical anion-exchange HPLC of recombinant type-5 adenoviral particles.. Hum. Gene Ther. 8: 453-465, 1997. PubMed: 9054520 61235: Lehmberg E, et al. Reversed-phase high-performance liquid chromatographic assay for the adenovirus type 5 proteome.. J. Chromatogr. B Biomed. Sci. Appl. 732: 411-423, 1999. PubMed: 10517364 61236: Obenauer-Kutner LJ, et al. The use of field emission scanning electron microscopy to assess recombinant adenovirus stability. Hum. Gene Ther. 13: 1687-1696, 2002. PubMed: 12396622 61238: Callahan JD. Statistical analysis of adenovirus reference material assay results: determination of particle concentration and infectious titer.. La Jolla, CA: Callahan Associates Inc.; 2002.
