Classification Adenoviridae, Mastadenovirus, Human adenovirus C
Deposited As Adenovirus type 5
Agent Human adenovirus 5
Strain Adenoid 75
Applications 用途 Viruscide testing Respiratory research
Biosafety Level 生物安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen 1 mL per vial
Storage Conditions 保藏溫度 -70°C or colder
Comments Not inactivated by ether. Contains soluble group-specific complement fixation antigen. Agglutinates rat red blood cells incompletely.
Effect on Host CPE, refractile rounding, clumping and sloughing
Recommended Host HeLa (ATCC CCL-2)
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent.
Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1).
Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-3 days Effect on Host CPE, refractile rounding, clumping and sloughing
Name of Depositor RJ Huebner, National Institutes of Health; M Shaffer, Public Health Research Institute of the City of New York
Special Collection NCRR Contract Source Spontaneously degenerating tissue culture of adenoid tissue from a 4-year-old girl with enlarged chronically infected tonsils, Washington DC, 1953
References 參考文獻(xiàn) Chemical disinfectants and antiseptics --- Application of European Standards for chemical disinfectants and antiseptics. London, UK:British Standards Institution;British Standard BS EN 14885:2006.
Chemical disinfectants and antiseptics --- Virucidal quantitative suspension test for chemical disinfectants and antiseptics used in human medicine --- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution;British Standard BS EN 14476:2005.
Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904
Huebner RJ, et al. Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc. Soc. Exp. Biol. Med. 84: 570-573, 1953. PubMed: 13134217
Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828 Rowe WP, et al. Studies of the adenoidal-pharyngeal-conjunctival (APC) group of viruses. Am. J. Hyg. 61: 197-218, 1955.
