ATCC? Number:VR-1737?
Classification: Orthomyxoviridae, Influenzavirus A
Agent: Influenza A virus (H1N1)
Strain: A/Virginia/ATCC2/2009
Original Source: Nasopharyngeal specimen from a clinical patient.
Depositors: ATCC
Biosafety Level:2
Shipped: frozen
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Host Organism : Production Host: MDCK (ATCCCCL-34)
Incubation : Plant cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI of about 0.1. Adsorb 1 hour at 35.0℃ in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. In Tissue culture, ATCCVR-1737 should be grown in serum free media that contains 2.0μg/ml TPCK-treated trypsin.
Effect : CPE in the form of rounded refractile cells with cell degeneration.
Store at : -70.0℃ or colder
Comments : This virus was isolated in tissue culture at ATCC.
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