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ATCC® Number:VR-1540™
Classification: Paramyxoviridae, Pneumovirus
Agent: Human respiratory syncytial virus deposited as Respiratory syncytial virus
Strain: A2
Original Source: Original isolate, deposited as VR-1302, was obtained from the lower respiratory tract of an infant with bronchiolitis and bronchopneumonia in Melbourne, Australia, 1961. VR-1540 was derived by treatment of VR-1302 to remove adenovirus contamination at ATCC.
Depositors: L Potash
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host:HEp-2 (ATCCCCL-23) Alternate Host(s): Monkey, HeLa, PrBEK, MRC-5, PrCK
Incubation : For best results, plate cells 24 to 48 hours in advance. Infect at about 80% confluence using a multiplicity of infection (MOI) of 0.1 to 1.0. Incubate 3 to 10 days at 37.0℃, until CPE are well advanced.
Effect : Yes, in vitro effects: Cytopathic effects in culture include syncytium formation by infected cells.
Store at : -70.0℃ or colder
Comments : VR-1540 was prepared from ATCCVR-1302 by growth in the presence of neutralizing serum to remove contaminating Adenovirus type 1.