亚日韩在线中文字幕亚洲,国产在线播放鲁啊鲁视频,张柏芝手扒性器全部图片,亚洲成无码电影在线观看

ATCC? Number:VR-1682?
Classification: Orthomyxoviridae, Influenzavirus A
Agent: Influenza A virus (H1N1)
Strain: A/Swine/1976/31 (TC-adapted)
Original Source: Hog in Iowa, 1931.
Depositors: M.T. Coleman and ATCC
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host: MDCK (ATCCCCL-34) Alternate Hosts: Chicken embryo; MDCK and monkey kidney cells (MkK)
Incubation : For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 35.0℃ in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. In TC, ATCCVR-1682 should be grown in serum free media that contains 1 ug/ml TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock).
Effect : CPE (cell rounding, degeneration, sloughing)
Store at : -70C℃ or colder
Comments : Derived by adaptation of egg-passage A/Swine/1976/31 (ATCCVR-99) to MDCK (ATCCCCL-34) cells. (Note: ATCCVR-99 and ATCCVR-1682 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture).