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平臺編號：bio-50976
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國際編號：ATCC VR-1679
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拉丁屬名： Influenza A virus H3N2
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務(wù)費用：
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
中文名稱暫無
ATCC? Number:VR-1679?
Classification: Orthomyxoviridae, Influenzavirus A
Agent: Influenza A virus (H3N2)
Strain: A/Hong Kong/8/68 (TC-adapted)
Original Source: Isolated from a patient in Hong Kong, 1968
Depositors: M. T. Coleman and ATCC
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host : MDCK (ATCCCCL-34) Alternate Hosts:CE, MDCK, MkK(monkey kidney cells)
Incubation : For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 35.0℃ in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. In TC, ATCCVR-1679 should be grown in serum free media that contains 1ug/ml TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock).
Effect : CPE (cell rounding, degeneration, sloughing)
Store at : -70℃ or colder
Comments : Derived by adaptation of egg-passage A/Hong Kong/8/68 (ATCCVR-544) to MDCK (ATCC-34) cells. (Note: ATCCVR-544 and ATCCVR-1679 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture.)
