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ATCC? Number:VR-1680?
Classification: Orthomyxoviridae, Influenzavirus A
Agent: Influenza A virus (H3N2)
Strain: A/Aichi/2/68 (TC-adapted)
Original Source: Isolated from sailor on Israeli ship docking at Aichi, Japan, 1968
Depositors: M.T. Coleman and ATCC
Biosafety Level:2
Shipped: frozen
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Host Organism : Production Host: MDCK (ATCCCCL-34) Alternate Host: CE, MDCK and Monkey kidney cells (MkK)
Incubation : For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 35.0℃ in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. In TC, ATCC廬 VR-1680 should be grown in serum free media that contains 1 ug/ml TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock).
Effect : CPE (cell rounding, degeneration, sloughing)
Store at : -70℃ or colder
Comments : Derived by adaptation of egg-passage A/Aichi/2/68 (ATCCVR-547) to MDCK (ATCCCCL-34) cells. (Note: ATCCVR-547 and ATCCVR-1680 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture).