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Clostridium perfringens (Veillon and Zuber) Hauduroy et al.拉丁名

(ATCC® 13124™)  統(tǒng)一編號(hào)

Strain Designations 菌種別名 NCTC 8237 [ATCC 19408, CIP 103 409, CN 1491, NCIB 6125, NCTC 6125, S 107]

Application 用途 Media testing

Produces hemolysins  Produces lethal toxins

Produces phospholipase C lecithinase C

Quality control strain

Reference material Control strain for anaerobe identification

Quality control strain for API, BBL, and MicroScan [Reg TM] products

Water testing
Type Strain 模式菌種 yes

iosafety Level 生物安全等級(jí) 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried

Storage Conditions 保藏方法

Frozen（冷凍物）: -80℃ or colder
Freeze-Dried（凍干物）: 2℃ to 8℃
Live Culture（活物）: See Propagation Section

Preceptrol® yes

Genome Sequenced Strain Clostridium perfringens ATCC 13124, complete genome.
Type Strain 模式菌種 yes

Comments 注釋 Penicillin-binding protein profil Type A, alpha-toxigenic The presence of cpa and pfoA genes was confirmed by PCR.

Medium培養(yǎng)基  ATCC® Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood

Growth Conditions 生長(zhǎng)條件‘’

Temperature 培養(yǎng)溫度: 37℃
Atmosphere 需氧情況: Anaerobic gas mixture, 80% N2-10% CO2-10% H2

Name of Depositor 來(lái)源國(guó)家 NCTC

Chain of Custody 來(lái)源國(guó)家ATCC <-- NCTC <-- Wellcome Lab. CN 1491
References 參考文獻(xiàn) J. Appl. Bacteriol. 22: 1, 1959.

Chalkley L, Van der Westhuyzen I. Penicillin-binding proteins of Clostridia. Curr. Microbiol. 26: 109-112, 1993.

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility (tests for specified micro-organisms). Nutritive and selective properties of the media and validity of the test for specified micro-organisms. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.13, 1997

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

US General Services Administration Test kit, anaerobe identification. Washington, DC:US General Services Administration;Commercial Item Description A-A-51238, 1985

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

API 20A Anaerobe. bioMerieux.

MicroScan Rapid Anaerobe ID QC Set. Dade/Siemens.

Water microbiology. Method 17.1: Spores of sulfite-reducing anaerobes (clostridia) including Clostridium perfringens --Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4276.17.1:2000.

Water microbiology. Method 17.2: Spores of sulfite-reducing anaerobes (clostridia) including Clostridium perfringens -- Estimation of most probable number (MPN) using the multiple tube dilution technique.. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4276.17.2:2000.

Water microbiology. Method 18: Aeromonas by membrane filtration including selected speciation. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4276.18:2001.

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

VITEK 2 ANC Comprehensive QC Set. bioMerieux.

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.

Cross References
Nucleotide (GenBank) : AX109537 Sequence 270 from Patent WO0123604.

Nucleotide (GenBank) : AX110098 Sequence 831 from Patent WO0123604.

Nucleotide (GenBank) : M59103 Clostridium perfringens 16S ribosomal RNA.

Nucleotide (GenBank) : J02880 C.perfringens pyruvoyl-dependent histidine decarboxylase (hdc), gene, complete cds.

Nucleotide (GenBank) : S51964 {16S/23S ribosomal DNA spacer region} [Clostridium perfringens, ATCC 13124, Genomic, 239 nt]

Nucleotide (GenBank) : CP00246 Clostridium perfringens ATCC 13124, complete genome.