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Penicillium rubens Biourge拉丁名 (ATCC® 9179™) 統(tǒng)一編號(hào)

Deposited As Penicillium notatum Westling

Strain Designations 菌株別名 NRRL 832 [ATCC 7813, B 69, CBS 197.46, IMI 17968, QM 940]

Application 用途 Produces penicillin； Produces phosphogluconate dehydrogenase 6-phosphogluconate dehydrogenase

Biosafety Level 生物安全等級(jí) 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Storage Conditions 保藏條件

Frozen 冷凍物: -80℃ or colder

Freeze-Dried 凍干物: 2℃ to 8℃

Live Culture 活物: See Propagation Section

Type Strain 模式菌株 no

Preceptrol® yes

Comments Prototype strain for production of penicillin in submerged culture The strain was reidentified as Penicillium rubens based on the multiloci DNA sequence analysis

Medium 培養(yǎng)基  ATCC® Medium 28: Emmons' modification of Sabouraud's agar

ATCC® Medium 312: Czapek's agar

ATCC® Medium 336: Potato dextrose agar (PDA)

Growth Conditions 生長(zhǎng)條件

Temperature 培養(yǎng)溫度: 25℃ to 30℃

Atmosphere 需氧情況: Typcial aerobic

Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCCAACCTCCCACCCGTGTTTATT

TTACCTTGTTGCTTCGGCGGGCCCGCCTTAACTGGCCGCCGGGGGGCTTACGCCCCCGGGCCCGCGCCCGCCGAAGA

CACCCTCGAACTCTGTCTGAAGATTGTAGTCTGAGTGAAAATATAAATTATTTAAAACTTTCAACAACGGATCTCTTGGTTCCG

GCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAAATTCAGTGAATCATCGAGTCTTTGAACGCACATTG

CGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTTCTGCCCTCAAGCACGGCTTGTGTGTTGGGCCCCGTC

CTCCGATCCCGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGGTCCTCGAGCGTATGGGGCTTTGTCACCC

GCTCTGTAGGCCCGGCCGGCGCTTGCCGATCAACCCAAATTTTTATCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCT

GAACTTAAGCATATCAATAA Beta-tubulin (TUB2) gene, partial sequence GATCCCCTCCAGACGCGTCTTTTGTTTTCATATGGCCCCTGAGCCACGACCCCACCCCCAACAGATCTTTTGCTAAC

TTGCTCTA

GGTTCACCTCCAAACCGGCCAGTGTGTAAGTTCAACATGGAACATTCTTGGAAACATTCTTCGATTCGTGGGACT

AAACTGGAATTGGGTTATAGGGTAACCAAATTGGTGCCGCTTTCTGGTAAGTCTCGGAGCTTTTTTTTTCGCGTTGGG

TATCAATTGACAAGTTGCTAACTGGATTACAGGCAAACCATCTCTGGCGAGCACGGTCTCGATGGCGATGGACAGTA

AGTTTAACAGTGATAGGGATTCTGGTGGATCACACGTCTGATATCTTGCTAGGTACAATGGTACCTCCGACCTCCAGC

TCGAGCGTATGAACGTCTACTTCAACCATGTGAGTACAATGACTGGGAATCGATTGATTGTGCATCATCTGATCGGAC

GTTTTTCTTTGACAATCTAGGCCAGCGGTGACAAGTACGTTCCCCGTGCCGTTCTGGTCGATTTGGAGCCCGGTACC

ATGGATGCTGTCCGCTCCGGTCCCTTCGGCAAGCTTTTCCGCCCCGACAACTTCGTCTTCGGTCAGTCCGGTGCTG

GTAACAACTGGGCCAAGGGTCACT D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATT

TGAAAGCTGGCTCCTTCGGGGTCCGCATTGTAATTTGTAGAGGATGCTTCGGGAGCGGTCCCCAT

CTAAGTGCCCTGGAACGGGACGTCATAGAGGGTGAGAATCCCGTATGGGATGGGGTGTCCGCGCCCGTGTGAAGC

TCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATATTGGCCGG

AGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAAAAGCACGTGAAATTG

TTGAAAGGGAAGCGCTTGCGACCAGACTCGCTCGCGGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCCGCGG

GCGGGCCAGCGTCGGTTTGGGCGGTCGGTCAAAGGCCCTCGGAAGGTAACGCCCCTAGGGGCGTCTTATAGCCG

AGGGTGCAATGCGACCTGCCTAGACCGAGGAACGCGCTTCGGCTCGGACGCTGGCATAATGGTCGTAAACGAC

Name of Depositor 寄存人 NRRL

Chain of Custody 來源國(guó)家 ATCC <-- NRRL <-- C Thom B 69

Isolation 分離基物 Contaminant in culture labeled 'P. lacticus Maze & Perrier' from Ph. Biourge 69, Washington, DC

Cross References  Nucleotide (GenBank) : KU729086 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : KU729184 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : KU896998 beta-tubulin (TUB2) gene

References參考文獻(xiàn) Houbraken J, et al. New penicillin-producing Penicillium species and an overview of section Chrysogena. Persoonia 29: 78-100, 2012. PubMed: 23606767

Miller HM, Shepherd MG. Purification and properties of 6-phosphogluconate dehydrogenase from Penicillium duponti and Penicillium notatum. Can. J. Microbiol. 18: 1289-1298, 1972. PubMed: 5052896

Moyer AJ, Coghill RD. Penicillin. IX. The laboratory scale production of penicillin in submerged cultures by Penicillium notatum Westling (NRRL 832). J. Bacteriol. 51: 79-93, 1946.

Chou H, et al. Transaldolases are novel and immunoglobulin E cross-reacting fungal allergens. Clin. Exp. Allergy 41: 739-749, 2011. PubMed: 21488999

Houbraken J, Frisvad JC, Samson RA. Fleming's penicillin producing strain is not Penicillium chrysogenum but P. rubens. IMA Fungus 2: 87-95, 2011. PubMed: 22679592

Chow LP, et al. Characterization of Pen n 13, a major allergen from the mold Penicillium notatum. Biochem. Biophys. Res. Commun. 269: 14-20, 2000. PubMed: 10694469