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YIp26 [pRB9]
    YIp26 [pRB9]
  • 平臺編號:bio-72681
  • 國際編號:ATCC 37057
  • 拉丁屬名: YIp26 [pRB9]
  • 規(guī)格:freeze-dried
  • 用途:ATCC原裝進(jìn)口
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

YIp26 [pRB9]

(ATCC? 37057?)

Designations YIp26 [pRB9]

Depositors D Botstein

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information Size (kb): 7.8000001907348630; Vector: YIp26 (plasmid) ;Construction: pBR322, Saccharomyces cerevisiae ;Marker(s):LEU2,URA3,ampR ;Construct size (kb): 7.800000190734863 ;Features: marker(s): ampR, LEU2, URA3; replicon: pMB1

Applications YI-type (integrating) shuttle vector integrating vector shuttle vector

Comments A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector. Orientations are such that transcription from both URA3 and LEU2 is counterclockwise. A 1.1 kb HindIII fragment containing URA3 and a 2.2 kb SalI/XhoI fragment containing LEU2 were cloned into the HindIII and SalI sites, respectively, of pBR322. The unique restriction sites are in the 3' untranslated regions of URA3(SmaI) and LEU2(SalI).

Media ATCC? Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0℃

References Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped freeze-dried


ATCC37057

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