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YIp26 [pRB9]

(ATCC? 37057?)

Designations YIp26 [pRB9]

Depositors D Botstein

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information Size (kb): 7.8000001907348630； Vector: YIp26 (plasmid) ；Construction: pBR322, Saccharomyces cerevisiae ；Marker(s):LEU2,URA3,ampR ；Construct size (kb): 7.800000190734863 ；Features: marker(s): ampR, LEU2, URA3； replicon: pMB1

Applications YI-type (integrating) shuttle vector integrating vector shuttle vector

Comments A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector. Orientations are such that transcription from both URA3 and LEU2 is counterclockwise. A 1.1 kb HindIII fragment containing URA3 and a 2.2 kb SalI/XhoI fragment containing LEU2 were cloned into the HindIII and SalI sites, respectively, of pBR322. The unique restriction sites are in the 3' untranslated regions of URA3(SmaI) and LEU2(SalI).

Media ATCC? Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0℃

References Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped freeze-dried