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Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz 拉丁名

(ATCC? 51299?) 統(tǒng)一編號

Strain Designations 菌種別名: NJ-3 /

Type Strain 模式菌種 : no /

Biosafety Level 生物安全等級 : 2

Strain Designations 菌種別名  NJ-3

Application 用途 Quality control strain Susceptibility disc testing

Susceptibility testing Isolation Peritoneal fluid, St. Louis, MO

Biosafety Level 生物安全等級 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Storage Conditions 保藏方法

Frozen（冷凍物）: -80℃ or colder

Freeze-Dried（凍干物）: 2℃ to 8℃

Live Culture（活物）: See Propagation Section

Preceptrol? yes Genotype vanB ant(6)-I aac(6') aph(2"")

Type Strain 模式菌株 no

Antibiotic Resistance Low-level vancomycin-resistant, VanB Resistant to Vancomycin. Sensitive to Teichoplanin. Comments Presence of vanB confirmed by PCR Ref

Medium 培養(yǎng)基 ATCC? Medium 2174: Brain heart infusion medium with vancomycin 4 mcg/ml

Growth Conditions  生長條件

Temperature 培養(yǎng)溫度 : 37℃

Atmosphere 需氧情況: Aerobic

Genotype vanB ant(6)-I aac(6') aph(2"")

Name of Depositor 寄存人  FC Tenover Chain of Custody ATCC <-- FC Tenover <-- J Hoppe-Bauer

Isolation 分離源  Peritoneal fluid, St. Louis, MO

References  參考文獻 Tenover FC, et al. Ability of clinical laboratories to detect antimicrobial agent- resistant enterococci [published erratum appears in J. Clin. Microbiol. 31: 3081, 1993]. J. Clin. Microbiol. 31: 1695-1699, 1993. PubMed: 8349745

Swenson JM, et al. Molecular characterization and multilaboratory evaluation of Enterococcus faecalis ATCC 51299 for quality control of screening tests for vancomycin and high-level aminoglycoside resistance in enterococci. J. Clin. Microbiol. 33: 3019-3021, 1995. PubMed: 8576364

Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3. Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007

BD Phoenix Gram Positive AST PMIC/ID QC Set. BD Becton Dickinson; Phoenix MicroScan Dried Gram Positive Panel QC Set. Dade/Siemens.

Sensititre? MIC Susceptibility. Thermo Scientific? Sensititre? Systems, Trek Diagnostic Systems.

VITEK 2 AST-GP QC Set. bioMerieux.

MicroScan Synergies plus Gram Positive QC Set. Dade/Siemens.

BD GeneOhm VanR QC Set. BD Becton Dickinson.

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.

Patel R, et al. Multiplex PCR Detection of vanA, vanB, vanC-1, and vanC-2/3 Genes in Enterococci. J. Clin. Microbiol. 35(3): 703-707, 1997. PubMed: 9041416