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美國(guó)ATCC>
ATCC菌種類>
pDMRpDMR
(ATCC? 77093?)
Designations pDMR
GenBank Number M23412
Species Drosophila melanogaster, fruit fly
Depositors JC Venter Vector Construct size (kb): 7.800000190734863 Insert DNA: cDNA
Insert lengths(kb): 4.800000190734863
Gene product: cholinergic receptor, muscarinic [CHRM*]
Insert Size (kb) 4.800
Media ATCC? Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments Restriction digests of the clone give the following sizes (kb): PstI--3.8, 3.5, 0.6; BamHI--6.0, 2.4, 0.3; HindIII--6.3, 1.6; SmaI--7.8; EcoRI/SmaI--7.8; EcoRI--7.8; EcoRI/PstI--3.4, 3.0, 0.6 (doublet), 0.3. The insert contains the following restriction sites (nt position according to the published sequence): PstI--434, 700, 1210, 1711; BamHI--2596; HindIII--3316. A full-length cDNA clone which includes approximately 0.3 kb 5' and 2.1 kb 3' untranslated sequence. Constructed by blunt-ending an EcoRI fragment before inserting it into the vector.
References Onai T, et al. Chromosome localization of a Drosophila muscarinic cholinergic receptor. FEBS Lett. 255: 219-225, 1989. PubMed: 2507354
J C Venter, personal communication