Clostridium difficile 拉丁名
(Hall and O'Toole) Prevot
(ATCC? 43255?) 菌株編號
Permits And Restrictions View Permits
Strain Designations 菌株別名 VPI 10463
Application Enteric Research
Isolation 分離源 Abdominal wound
Biosafety Level 生物安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions Frozen 保藏方法 : -80℃ or colder
Freeze-Dried 凍干物 : 2℃ to 8℃
Live Culture 活菌 : See Propagation Section
Preceptrol? no
Genotype Toxinotype: 0 Images Micrograph of Cells at 1000X ATCC 43255
Type Strain 模式菌株 no
Comments 注釋 Presence of tcdA and tcdB genes confirmed by PCR.
Ref Toxinotype 0 Binary toxin gene cdtB not amplified by PCR Ribotype 087 Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Medium 培養(yǎng)基 ATCC? Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
ATCC? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 37℃
Atmosphere 需氧情況 : Anaerobic gas mixture, 80% N2-10% CO2-10% H2 or 80% N2-20% CO2
Genotype Toxinotype: 0
Name of Depositor TD Wilkins, DM Lyerly
Chain of Custody 來源國家 ATCC <-- TD Wilkins, DM Lyerly <-- G Nygaard
Isolation 分離源 Abdominal wound
References 參考文獻(xiàn) Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
BD GeneOhm Cdiff. BD Becton Dickinson. Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999
Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN).
An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986
Kink JA, Williams JA. Antibodies to recombinant Clostridium difficile toxins A and B are an effective treatment and prevent relapse of C. difficile-associated disease in a hamster model of infection. Infect. Immun. 66: 2018-2025, 1998. PubMed: 9573084
