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Galactomyces candidus de Hoog et Smith 拉丁名

(ATCC? 34614?) 菌株編號

Permits and Restrictions View Permits

Deposited As Geotrichum candidum Link : Persoon

Application Degrades sauerkraut brine Produces acetoin dehydrogenase diacetyl reductase

Produces lactate dehydrogenase Produces lipase Produces polygalacturonase endopolygalacturonase, pectinase Quality control strain

Produces lipases I and II Produces type A lipase Produces extracellular lipase from sauerkraut brine Produces acetoin dehydrogenase from sauerkraut brine

Biosafety Level 生物安全等級 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format  提供形式 freeze-dried

Storage Conditions Frozen 保藏方法 : -80℃ or colder

Freeze-Dried 凍干物 : 2℃ to 8℃

Live Culture 活菌 : See Propagation Section

Type Strain 模式菌株 no

Preceptrol? yes

Comments 注釋 Crystal structure of lipase cDNA clone of lipase genes The strain was recently re-identified as Galactomyces candidus (anamorphic synonym: Geotrichum candidum) based on the multigene sequence analysis.

Morphology On YM medium at 25℃ after 3 days, colonies white to dingy white, finely hairy (able to see the branching hyphae in the texture), dense, raised.

Hyphae hyaline, guttulate. Arthroconidia rectangular to cubic, hyaline, smooth, 6-7.5 X 3-3.75 μm.

Yeast cells subglobose to oval, hyaline, smooth, larger cells 9-12 X 4.5-8.25 μm, smaller cells 5.25-6 X 4.5-5.25 μm.

Medium  培養(yǎng)基 ATCC? Medium 325: Malt extract agar (Blakeslee's formula)

ATCC? Medium 200: YM agar or YM broth

ATCC? Medium 1245: YEPD

Growth Conditions 生長條件 

Temperature 培養(yǎng)溫度 : 24℃ to 26℃

Atmosphere 需氧情況 : Typical aerobic

Name of Depositor M Iwai

Isolation 分離源 Clotted carrot, Japan

Cross References Nucleotide (GenBank) : KU933438 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : KU933412 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : KU933403 actin gene

Nucleotide (GenBank) : E02497 cDNA encoding Geotrichum candidum lipase.

Nucleotide (GenBank) : U02622 Geotrichum candidum ATCC 34614 lipase gene, partial cds.

Nucleotide (GenBank) : U26858 Galactomyces geotrichum isolate g56 28S rRNA gene, partial

Nucleotide (GenBank) : U02541 Geotrichum candidum ATCC 34614 lipase II gene, partial cds.

References  參考文獻(xiàn) . . Food Chem. 45: 15-17, 1992.

Hang YD, Woodams EE. Production and characterization of polygalacturonase from Geotrichum candidum. World J. Microbiol. Biotechnol. 8: 480-482, 1992.

Jacobsen T, Poulsen OM. Comparison of lipases from different strains of the fungus Geotrichum candidum. Biochim. Biophys. Acta 1257: 96-102, 1995. PubMed: 7619863

Shimada Y, et al. cDNA molecular cloning of Geotrichum candidum lipase. J. Biochem. 106: 383-388, 1989. PubMed: 2481674

Nagao T, et al. Cloning and sequencing of two chromosomal lipase genes from Geotrichum candidum. J. Biochem. 113: 776-780, 1993. PubMed: 8370674

Schrag JD, et al. Multiple crystal forms of lipases from Geotrichum candidum. J. Mol. Biol. 220: 541-543, 1991. PubMed: 1870119 . . Bioresour. Technol. 43: 181-183, 1993.

Hata Y, et al. Low resolution crystal structure of lipase from Geotrichum candidum (ATCC34614). J. Biochem. 86: 1821-1827, 1979. PubMed: 575138

Sugihara A, et al. Separation and characterization of two molecular forms of Geotrichum candidum lipase. J. Biochem. 107: 426-430, 1990. PubMed: 2341377

Shimada Y, et al. cDNA cloning and characterization of Geotrichum candidum lipase II. J. Biochem. 107: 703-707, 1990. PubMed: 2398037

Catoni E, et al. Overexpression of lipase A and B of Geotrichum candidum in Pichia pastoris: high-level production and some properties of functional expressed lipase B. Biotechnol. Tech. 11: 689-695, 1997.

Iwai M, et al. Lipid requirement for the lipase production by Geotrichum candidum Link. Agric. Biol. Chem. 37: 929-931, 1973.

Iwai M, et al. Synthesis of terpene alcohol esters by lipase. Agric. Biol. Chem. 44: 2731-2732, 1980.

Hang YD, Woodams EE. Lipase production by Geotrichum candidum from sauerkraut brine. World J. Microbiol. Biotechnol. 6: 418-421, 1990.

FF MicroPlate. Biolog. YT MicroPlate QC Set. Biolog. Arteau M, Labrie S, Roy D. Terminal-restriction fragment length polymorphism and automated ribosomal intergenic spacer analysis profiling of fungal communities in Camembert cheese. Int. Dairy J. 20: 545-554, 2010.