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Rhizopus oligosporus Saito菌株拉丁名

(ATCC 22959)菌株編號(hào)

Permits and Restrictions View Permits

Strain Designations菌株別名 NRRL 2710 [CBS 338.62, IFO 8631, IMI 174457, Scholer M 140]

Application Degrades phytic acid Produces acid protease protease (acid) Produces ergostadientriols

Produces ethyl alcohol ethanol Produces fumaric acid Produces lipase Produces oncom Produces tempeh

Produces ergostadientriols useful in lowering serum cholesterol levels Produces extracellular lipase Produces an antibiotic

Biosafety Level生物安全等級(jí) 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format提供形式 freeze-dried

Storage Conditions Frozen保藏方法: -80℃ or colder

Freeze-Dried凍干物: 2℃ to 8℃

Live Culture活菌: See Propagation Section

Type Strain模式菌株 no

Preceptrol? no

Comments注釋 The current accepted  name of Rhizopus oligosporus is Rhizopus microsporus var. oligosporus (Saito) Schipper et Stalpers Morphology After 7 days on PDA at 25℃, colonies white, cottony, reverse uncolored.

Sporangia branched, heads globose, columellae subglobose or short-pyriform.

Sporangiospores hyaline to light gray, surface ornamentation not observed.

Medium培養(yǎng)基 ATCC Medium 324: Malt extract agar

ATCC Medium 335: Potato carrot agar

ATCC Medium 336: Potato dextrose agar (PDA)

Growth Conditions生長(zhǎng)條件

Temperature培養(yǎng)溫度: 24℃ to 26℃

Atmosphere需氧情況: Typical aerobic

Name of Depositor NRRL

Cross References交叉文獻(xiàn)Nucleotide (GenBank) : KU729104 ITS including 5.8S rRNA gene Nucleotide (GenBank) : KU729205 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : KU933450 RNA polymerase II largest subunit (RPB1) gene References Wang HL, et al. Acid protease production by fungi used in soybean food fermentation. Appl. Microbiol. 27: 906-911, 1974. PubMed: 4857353

Wang HL, Hesseltine CW. Studies on the extracellular proteolytic enzymes of Rhizopus oligosporus. Can. J. Microbiol. 11: 727-732, 1965. PubMed: 5861291

Nahas E. Control of lipase production by Rhizopus oligosporus under various growth conditions. J. Gen. Microbiol. 134: 227-233, 1988.

Nakashima T, et al. Cell aggregation as a trigger for enhancement of intracellular lipase production by a Rhizopus species. J. Ferment. Bioeng. 70: 85-89, 1990.

Zilliken FW. Ergostadienetriols, compositions containing same, and methods of preparing and using same. US Patent 4,234,577 dated Nov 18 1980 Sourcebook of experiments for the teaching of microbiology. London: Academic Press; 1982.

Kobayasi S, et al. Purification and characterization of an antibiotic substance produced from Rhizopus oligosporus IFO 8631. Biosci. Biotechnol. Biochem. 56: 94-98, 1992. PubMed: 1368137

. . Soybean Dig. Nov: 14-15, 1961. Djien KS, Hesseltine CW. Made from soybeans Indonesian fermented foods. Soybean Dig. 22: 14-15, 1961.

Fardiaz D, Markakis P. Degradation of phytic acid in oncom (fermented peanut press cake). J. Food Sci. 46: 523-525, 1981.

Soccol CR, et al. Production of L-lactic acid by Rhizopus species. World J. Microbiol. Biotechnol. 10: 433-435, 1994.

Walther G, et al. DNA barcoding in Mucorales: an inventory of biodiversity. Persoonia 30: 11-47, 2013. PubMed: 24027345

Schoch CL, et al. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci USA 109: 6241-6246, 2012. PubMed: 22454494

Liou G-Y, et al. Polyphasic approach to the taxonomy of the Rhizopus stolonifer group. Mycol Res 111: 196-203, 2007. PubMed: 17324757

Abe A, et al. The molecular phylogeny of the genus Rhizopus based on rDNA sequences. Biosci Biotechnol Biochem 70: 2387-2393, 2006. PubMed: 17031057

Yamada O, et al. Cloning and heterologous expression of the antibiotic peptide (ABP) genes from Rhizopus oligosporus NBRC 8631. Biosci Biotechnol Biochem 69: 477-482, 2005. PubMed: 15784974

Keeling PJ. Congruent evidence from alpha-tubulin and beta-tubulin gene phylogenies for a zygomycete origin of microsporidia. Fungal Genet Biol 38: 298-309, 2003. PubMed: 12684019

O'Donnell K, et al. Evolutionary relationships among mucoralean fungi (Zygomycota): Evidence for family polyphyly on a large scale. Mycologia 93: 286-296, 2001.

Voigt K, Wostemeyer J. Phylogeny and origin of 82 zygomycetes from all 54 genera of the Mucorales and Mortierellales based on combined analysis of actin and translation elongation factor EF-1alpha genes. Gene 270: 113-120, 2001. PubMed: 11404008

Tanabe Y, et al. Molecular phylogeny of parasitic zygomycota (Dimargaritales, zoopagales) based on nuclear small subunit ribosomal DNA sequences. Mol Phylogenet Evol 16: 253-262, 2000. PubMed: 10942611

Keeling PJ, Luker MA, Palmer JD. Evidence from beta-tubulin phylogeny that microsporidia evolved from within the fungi. Mol Biol Evol 17: 23-31, 2000. PubMed: 10666703