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Encephalomyocarditis virus拉丁名

(ATCC® VR-1763™)菌株編號(hào)

Classification分類: Picornaviridae, Cardiovirus /

Product Format提供形式: frozen

1 mL per vial Classification Picornaviridae, Cardiovirus Agent Encephalomyocarditis virus Strain MM

Biosafety Level生物安全等級(jí) 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format提供形式 frozen

1 mL per vial

Storage Conditions保藏方法 -70℃ or colder

Images VR-1763

Comments注釋 This item was deposited at ATCC® after six passages in weanling mice and three passages in suckling mice. A seed stock was produced by a single passage in BHK-21 cells. Effect on Host CPE, refractile rounding and sloughing Recommended Host BHK-21 (ATCC® CCL-10™)

Growth Conditions生長(zhǎng)條件

Temperature培養(yǎng)溫度: 37℃

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-95% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01).

Adsorb 1-2 hours at 37℃ in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 1-3 days Effect on Host CPE, refractile rounding and sloughing