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平臺編號：bio-76597
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國際編號：ATCCVR-1479
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拉丁屬名： Encephalomyocarditis virus
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規(guī)格：Frozen
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用途：ATCC原裝進口
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
中文名暫無
Encephalomyocarditis virus拉丁名
(ATCC® VR-1479™)菌株編號
Classification分類: Picornaviridae, Cardiovirus /
Product Format提供形式: frozen
Classification Picornaviridae, Cardiovirus Agent Encephalomyocarditis virus Strain M-variant (TC passaged)
Biosafety Level 生物安全等級2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format提供形式 frozen
Storage Conditions保藏方法 -70℃ or colder
Comments注釋 ATCC® VR-1479™ (M-varient) is a cell culture passaged linage of the M-varient (ATCC® VR-1314™) reported to induce diabetes in selected strains of mice such as Balb/cBy and DBA/2 mice.
Note that virus loses diabetagenic properties upon multiple passage in cell culture. Effect on Host Rounding and sloughing with general degeneration Recommended Host NCTC Clone 929 (ATCC® CCL-1™)
Growth Conditions生長條件
Temperature培養(yǎng)溫度: 37℃
Recommendations for Infection: For best results, infection should be performed on a 75-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended).
Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1 hour at 37℃ in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-10 days Effect on Host Rounding and sloughing with general degeneration
