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Encephalomyocarditis virus拉丁名

(ATCC® VR-1479™)菌株編號(hào)

Classification分類: Picornaviridae, Cardiovirus /

Product Format提供形式: frozen

Classification Picornaviridae, Cardiovirus Agent Encephalomyocarditis virus Strain M-variant (TC passaged)

Biosafety Level 生物安全等級2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format提供形式 frozen

Storage Conditions保藏方法 -70℃ or colder

Comments注釋  ATCC® VR-1479™ (M-varient) is a cell culture passaged linage of the M-varient (ATCC® VR-1314™) reported to induce diabetes in selected strains of mice such as Balb/cBy and DBA/2 mice.

Note that virus loses diabetagenic properties upon multiple passage in cell culture. Effect on Host Rounding and sloughing with general degeneration Recommended Host NCTC Clone 929 (ATCC® CCL-1™)

Growth Conditions生長條件

Temperature培養(yǎng)溫度: 37℃

Recommendations for Infection: For best results, infection should be performed on a 75-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended).

Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1 hour at 37℃ in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-10 days Effect on Host Rounding and sloughing with general degeneration