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Aureobasidium pullulans 菌株拉丁名(de Bary) Arnaud

(ATCC 9348) 菌株編號

Strain Designations 菌株別名: F-44 [CBS 621.80, DSM 2404, IMI 145194, QM 3090]  /

Product Format 提供形式: freeze-dried

Deposited As Pullularia pullulans (de Bary) Berkhout

Application Bacterial resistance testing adhesives 

Degrades plastics 

Fungus resistance testing 

Fungus resistance testing adhesives 

Fungus resistance testing lacquer 

Fungus resistance testing paint 

Fungus resistance testing plastics 

Fungus resistance testing textiles 

Fungus resistance testing varnish 

Produces inulosucrase fructosyl transferase 

Produces pullulan 

Testing

Biosafety Level 生物安全等級 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Storage Conditions 保藏方法

Frozen 冷凍物: -80℃ or colder

Freeze-Dried 凍干物: 2℃ to 8℃

Live Culture 活菌: See Propagation Section

Type Strain 模式菌株 no 

Medium 培養(yǎng)基 ATCC Medium 340: Rabbit food agar 

ATCC Medium 336: Potato dextrose agar (PDA) 

ATCC Medium 28: Emmons' modification of Sabouraud's agar 

Growth Conditions 生長條件

Temperature 培養(yǎng)溫度: 24°C to 26°C 

Atmosphere 需氧情況: Typical aerobic

Preceptrol no

Comments 注釋 The DNA barcode sequences indicate that this fungus is close to Aureobasidium pullulans var. melanogenum (= A. melanigenum). Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGTAAGGGTGCTCAGCGCCCGACCTCCAACC

CTTTGTTGTTAAAACTACCTTGTTGCTTTGGCGGGACC

GCTCGGTCTCGAGCCGCT

GGGGATTCGTCCCAGGCGAGCGCCCGCC

AGAGTTAAACCAAACTCTTGTTATTTAACCGGTCGTCTGAGTTAAAATTTTGAATAAATCAAAACTTTCAACAA

CGGATCTCTTGGTTCTCGCATCGATGAA

GAACGCAGCGAAATGCGATAAG

TAATGTGAATTGCAGAATTCAGTGAATCATCGAATCT

TTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTA

CACCACTCAAGCTATGCTTGGTATTGGGTGCC

GTCCTTAGTTGGGCGCGCCTTAAAGACCTCGGC

GAGGCCTCACCGGCTTTAGGCGTAGTAGAATTTA

TTCGAACGTCTGTCAAAGGAGAGGACTTCTGCCGACTGAAACCTTTATTTTTCTAGGTTGACCTCGGATCAGGT

AGGGATACCCGCTGAACTTAAGCATATCAATAA     

D1D2 region of the 28S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTC

AAATTTGAAAGCTGGCC

TTCGGGTCCGCATTGTAATTTGTAGAGGATGC

TTTGGGTGAAACGCCAGTCTAAGTTCCTTGGAAC

AGGACGTCATAGAGGGTGAGAATCCCGTATGTGACTGGAAATGTTAACCTATGTAAAGCTCCTTCGACGA

GTCGAGTTGTTTGGGAATGC

AGCTCTAAATGGGAGGTAAATTTCTTCTAAAG

CTAAATATTGGCGAGAGACCGATAGCGCACAAGTA

GAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAAGCACGTGAAATTGTTGAAAGGGAAGC

GCTTGCAATCAGACTTG

TTTAAACTGTTCGGCCGGTCTTCTGACCGGTTTA

CTCAGTTTGGACAGGCCAGCATCAGTTTCGGCGGC

CGGATAAAGGCTCTGGGAATGTGGCCTTCACTTCGGTGAAGGTGTTATAGCCCAGGGTGTAATAC

GGCCAGCCGGGACTG

AGGTCCGCGCTTCGGCTAGGATGCTGGCGTAATGGTTGTAAGCGAC

Name of Depositor 寄存人 PB Marsh

Chain of Custody 來源國家 ATCC <-- PB Marsh <-- WL White F-44 <-- GF Weber

Isolation 分離源 Deteriorated army supplies, Florida Cross

References 參考文獻 Nucleotide (GenBank) : KU933441 ITS including 5.8S rRNA gene  

Nucleotide (GenBank) : KU933415 D1/D2 region of 28S rRNA gene   

References Catley BJ. The extracellular polysaccharide, pullulan, produced by Aureobasidium pullulans: a relationship between elaboration rate and morphology. J. Gen. Microbiol. 120: 265-268, 1980.   

Heady RE.

Process for the production of high fructose syrups and ethanol. US Patent 4,356,262 dated Oct 26 1982   

Heady RE. Preparation of high fructose syrups from sucrose. US Patent 4,276,379 dated Jun 30 1981   

Bock A, et al.

Aureobasidium pullulans strain, process for its preparation, and use thereof. US Patent 5,019,514 dated May 28 1991   

ASTM International Standard Test Method for Resistance to Growth of Mold on the Surface of Interior Coatings in an Environmental Chamber. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 3273-00 (Reapproved 2005).

ASTM International Standard Test Methods for Ability of Adhesive Films to Support or Resist the Growth of Fungi. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4300-01.   

ASTM International Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4783-01e1.

Deutsches Institut fur Normung Testing of plastics: Influence of fungi and bacteria; visual evaluation, change in mass or physical properties. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 53739.   

IEC Basic environmental testing procedures: Mould growth. Geneva (Switzerland):International Electrotechnical Commission;IEC 60068-2-10, Part 2, Test J.   Klausmeier RE.

Results of the second interlaboratory experiment of biodeterioration of plastics. Int. Biodeterior. Bull. 8: 3-7, 1972.   

Catley BJ, Hutchinson A. Elaboration of pullulan by spheroplasts of Aureobasidium pullulans. Trans. Br. Mycol. Soc. 76: 451-456, 1981.West TP, Reed-Hamer B.

Effect of Ph on pullulan production relative to carbon source and yeast extract composition of growth medium. Microbios 75: 75-82, 1993.  

Environmental testing. Part 2.10: Tests--Test J and guidance: Mould growth. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 60068.2.10-2003.

Fibre Optic interconnecting divices and passive components --Basic test and measurement procedures--Part 2-16: Tests --Mould growth. Geneva (Switzerland):International Electrotechnical Commission;IEC 1300-2-16, 1995   

Plastics--Evaluation of the action of micro-organisms.

Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 846:1997.   

Testing of textiles -- Evaluation of the action of microfungi. London, UK:British Standards Institution;British Standard BS EN 14119:2003, 2003   Standard method of evaluating the resistance of wood product surfaces to mold growth.

Birmingham, AL: American Wood-Preservers' Association; AWPA E24-06, 2006  

Plastics - Evaluation of the action of microorganisms. London, UK:British Standards Institution;British Standard BS EN ISO 846:1997.

Loncaric I, et al. Phenotypic and genotypic diversity among strains of Aureobasidium pullulans in comparison with related species. Antonie van Leeuwenhoek 95: 165-178, 2009. PubMed: 19123008   

Zalar P, et al. Redefinition of Aureobasidium pullulans and its varieties. Stud Mycol 61: 21-38, 2008. PubMed: 19287524