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Cryptococcus neoformans var. grubii拉丁名

(ATCC? 208821?)菌株編號

Strain Designations菌株別名: H99 [H99JP, NYSD 1649] /

Product Format提供形式: frozen

 Permits and Restrictions View Permits Deposited As Cryptococcus neoformans (Sanfelice) Vuillemin

Strain Designations菌株別名： H99 [H99JP, NYSD 1649]

Application Biomedical Research and Development Material Emerging infectious disease research Negative control in differential gene expression studies Produces laccase

Biosafety Level生物安全等級 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 生長條件 frozen

Storage Conditions 保藏方法

Frozen: -80℃ or colder

Freeze-Dried凍干物: 2℃ to 8℃

Live Culture活菌: See Propagation Section

Type Strain模式菌株 yes Antigenic Properties Serotype A

Preceptrol? no

Genome Sequenced Strain Yes Mating Type Mating type: alpha (Peter R Williamson, personalcommunication)

Comments 注釋Multigene phylogeny and phenotypic characterization.

Facultative intracellular pathogen Genome sequencing strain (Broad Institute, USA) Morphology After 8 days on YM agar at 30℃, colony is cream-colored, smooth, mucoid. Cells globose or subglobose, single or with bud, pseudohyphae not observed.

Medium培養(yǎng)基 ATCC? Medium 28: Emmons' modification of Sabouraud's agar

ATCC? Medium200: YM agar or YM broth

 ATCC? Medium 324: Malt extract agar Growth Conditions

Temperature培養(yǎng)溫度: 25℃ to 30℃

Atmosphere: Typical aerobic Sequenced Data

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTCGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCTTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCGGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 28S ribosomal RNA gene.

CATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGGGGAATGTAGCACTCTTCGGAGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC

Morphology After 8 days on YM agar at 30℃, colony is cream-colored, smooth, mucoid. Cells globose or subglobose, single or with bud, pseudohyphae not observed.