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Escherichia coli (Migula) Castellani and Chalmers  拉丁名

(ATCC? 47055?) 統(tǒng)一編號

Classification 類別 Enterobacteriaceae, Escherichia

Strain Designations 菌種別名 S17-1 

Application 用途 transformation host

Biosafety Level 生物安全等級 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 frozen

Storage Conditions -80℃ or colder

Preceptrol? no

Genotype recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 integrated into the chromosome

Type Strain 模式菌株 no

Comments 注釋 Recommended mobilization host for pARO180, pARO190, pARO181, and pARO191 (ATCC 77123-77126, respectively) because it contains chromosomally integrated tra genes. A kanamycin-sensitive strain devoid of the E. coli K-12-specific restriction system allowing efficient uptake of foreign DNA. The properties of streptomycin, trimethoprim, and spectinomycin resistance were verified. RP4-Tc::Mu-Km::Tn7 is integrated into the chromosome.

Medium 培養(yǎng)基 ATCC? Medium 1065: LB Agar/Broth, Miller

Growth Conditions 生長條件

Temperature 培養(yǎng)溫度: 37℃

Atmosphere 需氧情況 : Aerobic

Genotype recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 integrated into the chromosome

Name of Depositor 寄存人 D Parke, Department of Biology, Yale University, New Haven, CT

Chain of Custody 來源國家 ATCC <-- D Parke <-- R Simon

References  參考文獻 McFarlane GJ, et al. A simplified method for conjugal gene transfer into the filamentous cyanobacterium Anabaena sp. ATCC 27893.

J. Microbiol. Methods 6: 301-305, 1987.

Parke D. Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990. PubMed: 2227423

Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983.