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Aspergillus fumigatus Fresenius 菌株拉丁名

(ATCC® 13073™) 菌株編號(hào)

Strain Designations 菌株別名 5233 [NIH 5233]

Application 用途

Produces antigens

Biomedical Research and Development Material

Emerging infectious disease research

Opportunistic pathogen research

Respiratory research

Biosafety Level 生物安全等級(jí) 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Storage Conditions 保藏方法 Frozen: -80℃ or colder

Freeze-Dried 凍干物 : 2℃ to 8℃

Live Culture 活菌 : See Propagation Section

Type Strain 模式菌株 no

Preceptrol® no

Comments 注釋 Toxic to human cancer cell lines

Morphology After 7-10 days on MEA at 25℃ mycelium white; reverse uncolored, yellowish, red brown or green.

Conidia grayish turquoise to dull green. Conidial heads predominantly columnar; stipes uncolored, smooth-walled. Conidia globose to broadly ovoid, smoothly to finely roughened.

Medium 培養(yǎng)基 ATCC® Medium 325: Malt extract agar (Blakeslee's formula)

ATCC® Medium 336: Potato dextrose agar (PDA)

ATCC® Medium 28: Emmons' modification of Sabouraud's agar

Growth Conditions 生長(zhǎng)條件 

Temperature 培養(yǎng)溫度 : 24℃ to 26℃

Atmosphere 需氧情況 : Typical aerobic

Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCCAACCTCCCACCCGTGTCTAT

CGTACCTTGTTGCTTCGGCGGGCCCGCCGTTTCGACGGCCGCCGGGGAGGCCTTGCGCCCCCGGGCCCGCGCC

CGCCGAAGACCCCAACATGAACGCTGTTCTGAAAGTATGCAGTCTGAGTTGATTATCGTAATCAGTTAAAACTTTCAACAA

CGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCAT

CGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAG

CACGGCTTGTGTGTTGGGCCCCCGTCCCCCTCTCCCGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCC

GGTCCTCGAGCGTATGGGGCTTTGTCACCTGCTCTGTAGGCCCGGCCGGCGCCAGCCGACACCCAACTTTATTTTTC

TAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA

Morphology After 7-10 days on MEAat 25℃ mycelium white; reverse uncolored, yellowish, red brown or green.

Conidia grayish turquoise to dull green. Conidial heads predominantly columnar; stipes uncolored, smooth-walled. Conidia globose to broadly ovoid, smoothly to finely roughened.

Name of Depositor 寄存人 HF Hasenclever

Isolation 分離源 Human pulmonary lesion, Maryland

Cross References Nucleotide (GenBank) : AY040608 Plasma membrane H+-ATPase (PMA1) gene, complete coding sequence.

Nucleotide (GenBank) : AY040609 Plasma membrane H+-ATPase (PMA1) gene, mRNA, complete coding sequence.

Nucleotide (GenBank) : KC689314 ITS including 5.8S rRNA gene

References  參考文獻(xiàn) Kim SJ, Chaparas SD. Characterization of antigens from Aspergillus fumigatus. I. Preparation of antigens from organisms grown in completely synthetic medium. Am. Rev. Respir. Dis. 118: 547-560, 1978. PubMed: 101103

Daly P, et al. Culture filtrates of Aspergillus fumigatus induce different modes of cell death in human cancer cell lines. Mycopathologia 146: 67-74, 1999.

Burghoorn HP, et al. Molecular evaluation of the plasma membrane proton pump from Aspergillus fumigatus. Antimicrob. Agents Chemother. 46: 615-624, 2002. PubMed: 11850239

Tuntevski K, et al. Aspergillus collagen-like (acl) genes: identification, sequence polymorphism and assessment for PCR-based pathogen detection. Appl. Environ. Microbiol. 79: 7882-7895, 2013. PubMed: 24123732

Jaques AK, et al. Disruption of the gene encoding the ChiB1 chitinase of Aspergillus fumigatus and characterization of a recombinant gene product. Microbiology 149: 2931-2939, 2003. PubMed: 14523125

Nascimento AM, et al. Multiple resistance mechanisms among Aspergillus fumigatus mutants with high-level resistance to itraconazole. Antimicrob Agents Chemother 47: 1719-1726, 2003. PubMed: 12709346