Eubacterium rectale (Hauduroy et al.) Prevot 菌株拉丁名
(ATCC? 33656?) 菌株編號
Strain Designations 菌株別名 VPI 0990 [CIP 105953]
Isolation 分離源 Feces, human
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏方法
Frozen 冷凍物 : -80℃ or colder
Freeze-Dried 凍干物 : 2℃ to 8℃
Live Culture 活菌 : See Propagation Section
Preceptrol? no
Type Strain 模式菌株 no
Comments 注釋 Referred to as the type strain
Medium 培養(yǎng)基 ATCC? Medium 1703: Chopped meat carbohydrates with rumen fluid
ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Handling procedure Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #1703 broth.
Aseptically transfer the entire contents to a 5-6 mL tube of #1703 broth.
Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
Best practice dictates the use of pre-reduced media. Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
Incubate in an anaerobic atmosphere at 37°C for 24-72 hours. Incubate one agar plate aerobically at 37°C to check for contamination.
ANAEROBIC CONDITIONS: Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following: Loose screw caps on test tubes in an anaerobic chamber Loose screw caps on test tubes in an activated anaerobic gas pack jar Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 37℃
Atmosphere 需氧情況 : Anaerobic gas mixture, 97% CO2-3% H2
Name of Depositor 寄存人 WE Moore
Isolation 分離源 Feces, human
References 參考文獻 Bergey's Manual Syst. Bacteriol. 2: 1368-1369, 1986.
Cross References Nucleotide (GenBank) : L34627 Eubacterium rectale 16S ribosomal RNA.