![WM179 [CBS 10078]](http://www.family998.com/static/images/no_picture.jpg)
Cryptococcus gattii (Vanbreuseghem et Takashio) Kwon-Chung et Boekhout
MYA-4560 ™
Download GenomeLearn about the ATCC Genome Portal An ampoule containing mycelia-medium cubes suspended in cryoprotectant.
Product categoryFungi
Product typeYeast
Strain designationWM179 [CBS 10078]Type strainNoGenome sequenced strainYesGeographical isolationAustraliaApplicationsAgricultural research
Product formatFrozenStorage conditions-80°C or colder
General
Specific applications
Biomedical Research and Development Material
Preceptrol
No
Characteristics
Serotype
B
Mating type
alpha
Genotype
VGI, AFLP4
Comments
Reference strain of VG I, AFLP4 molecular type of C. gattii.
Handling information
Medium
ATCC Medium 200: YM agar or YM broth
ATCC Medium 336: Potato dextrose agar (PDA)
ATCC Medium 325: Malt extract agar (Blakeslee's formula)
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
