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Aspergillus fijiensis Varga et al. 菌株拉丁名

(ATCC® 20611™)  統(tǒng)一編號

Deposited As Aspergillus niger van Tieghem

Strain Designations 菌株別名 ACE-2-1

Application 用途 produces inulosucrase fructosyl transferase produces pyridoxine derivatives produces a fructooligosaccharide-producing beta-fructofuranosidase

Biosafety Level 生物安全等級 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Storage Conditions 保藏方法 

Frozen 冷凍物 : -80℃ or colder

Freeze-Dried 凍干物 : 2℃ to 8℃

Live Culture 活菌 : See Propagation Section

Type Strain 模式菌株 no

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims.

Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.

This material may not have been produced or characterized by ATCC.

Preceptrol® no

Comments The strain was re-identified based on multigene sequence analyses.

Medium 培養(yǎng)基 ATCC® Medium 200: YM agar or YM broth

ATCC® Medium 336: Potato dextrose agar (PDA)

Growth Conditions 生長條件 

Temperature 培養(yǎng)溫度 : 24℃ to 26℃

Atmosphere 需氧情況 : Typical aerobic

Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCTGGGTCCTTCGGGGCCCAACCT

CCCACCCGTGCTTACCGTACCCTGTTGCTTCGGCGGGCCCGCCTTCGGGCGGCCCGGGGCCT

GCCCCCGGGACCGCGCCCGCCGGAGACCCCAATGGAACACTGTCTGAAAGCGTGCAGTCTGAG

TCGATTGATACCAATCAGTCAAAACTTTCAACAATGGATCTCTTGGTTCCGGCATCGATGAAGAACG

CAGCGAAATGCGATAACTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTG

CGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTTCTCCCCTCCAGCCCCGCTG

GTTGTTGGGCCGCGCCCCCCCGGGGGCGGGCCTCGAGAGAAACGGCGGCACCGTCCGGTCCT

CGAGCGTATGGGGCTCTGTCACCCGCTCTATGGGCCCGGCCGGGGCTTGCCTCGACCCCCAAT

CTTCTCAGATTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 28S ribosomal

RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTCAGTAACGGCGAGTGAAGCGGC

AAGAGCTCAAATTTGAAAGCTGGCCCCTCCGGGGTCCGCGTTGTAATTTGCAGAGGATGCTTT

GGGTGCGGCCCCCGTCTAAGTGCCCTGGAACGGGCCGTCAGAGAGGGTGAGAATCCCGTC

TTGGGCGGGGTGTCCGTGCCCGTGTAAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCA

GCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACTGGCCGGAGACCGATAGCGCACAAGT

AGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGCACGTGAAATTGTTGAAAGGG

AAGCGCTTGCGACCAGACTCGCCCTCGGGGTTCAGCCGGCACTCGTGCCGGTGTACTTCC

CCGGGGGCGGGCCAGCGTCAGCTTGGGCGGCCGGTCAAAGGCCTCCGGAATGTAGTGCC

CCCCGGGGCACCTTATAGCCGGAGGTGCAATGCGGCCAGCCTGGGCTGAGGAACGCGCTTC

GGCACGGACGCTGGCATAATGGTCGCAAACGAC Calmodulin gene TTTTTGTAAGCGACCCCGA

TGCTTTCTCGTCTGCTATAAAACAATGTTTACTGATTCGTTTCTCG

TGATCCCTGATAGGACAAGGATGGCGATGGTTAGTGTAGTTCTTTACCCCTCAACGAAACCCGC

CTGGCATCACCTGGCACTATAGCCACCATACCGCAAACGCCACAGAAACTATCAAGAATCGATCG

CATTTCTGACAGAATCCATGCTACAGGACAAATTACCACCAAGGAGTTGGGTACCGTTATGCGCTCT

CTCGGCCAGAACCCCTCCGAGTCTGAGCTCCAGGACATGATCAACGAGGTTGACGCTGACAACAA

CGGCACTATCGACTTTCCCGGTATGTGATGGCCATGCATCGAGCTCCGGACATAGTATTGACAGCTGCCCAGAATTCCTTA

Name of Depositor 寄存人 Meiji Seika Kaisha, Ltd. U.S.

Patent Number 4,681,771

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims.

Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.

This material may not have been produced or characterized by ATCC.

Cross References Nucleotide (GenBank) : KU729079 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : KU729176 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : KU933404 calmodulin gene

References  參考文獻(xiàn) Suzuki Y, Uchida K. Formation of beta-fructosyl compounds of pyridoxine in growing culture of Aspergillus niger. Biosci. Biotechnol. Biochem. 57: 875-880, 1993. PubMed: 7763873

Kono T, et al. Fructose transferring enzyme absorbed on a granular carrier for production of fructooligosaccharides. US Patent 5,314,810 dated May 24 1994

Adachi T, Hidaka H. Sweetener. US Patent 4,681,771 dated Jul 21 1987

Hidaka H, et al. A fructooligosaccharide-producing enzyme from Aspergillus niger ATCC 20611.Agric. Biol. Chem. 52: 1181-1187, 1988.

Hirayama M, et al. Purification and properties of a fructooligosaccharide-producing beta-fructofuranosidase from Aspergillus niger ATCC 20611. Agric. Biol. Chem. 53: 667-673, 1989.