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pMMB190
    pMMB190
  • 平臺(tái)編號(hào):bio-81724
  • 國際編號(hào):ATCC 37807*
  • 拉丁屬名: pMMB190
  • 規(guī)格:freeze-dried
  • 用途:ATCC原裝進(jìn)口
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

pMMB190 統(tǒng)一拉丁名

(ATCC? 37807?) 統(tǒng)一編號(hào) 

Designations pMMB190 

Depositors VM Morales 

Biosafety Level 生物安全等級(jí) 1 

Vector Information

Size (kb): 9.0640001296997070 

Vector: pMMB190 (plasmid) 

Promoters: Promoter tac 

Construction: pMMB66EH, pUC8 

Marker(s):ampR 

Construct size (kb): 9.064000129699707 

Features: insert detection: lacZ' marker(s): ampR promoter: tac, lacUV5 replicon: oriV, oriT (IncQ) repressor gene: lacIq transcription terminator: rrnB 

Applications 用途 expression vector shuttle vector 

Comments 注釋 Restriction digests of the clone give the following sizes (kb): EcoRI--9.1; EcoRV--5.3, 3.8; SspI--4.9, 1.8, 1.6, 1.0; EcoRI/MluI--8.2, 0.9. 

Contains sequences encoding lacIq, a tandem tac lacUV5 promoter region, and lacZalpha to allow direct screening by inactivation of alpha-complementation. 

Shows a low level of expression without induction. 

Media ATCC? Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin 

Growth Conditions 生長條件 

Temperature 培養(yǎng)溫度 : 37.0°C 

References 參考文獻(xiàn) Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347 

Shipped freeze-dried Shipping Information Shipped:  Freeze dried E. coli  containing the plasmid.


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