pMMB190 統(tǒng)一拉丁名
(ATCC? 37807?) 統(tǒng)一編號(hào)
Designations pMMB190
Depositors VM Morales
Biosafety Level 生物安全等級(jí) 1
Vector Information
Size (kb): 9.0640001296997070
Vector: pMMB190 (plasmid)
Promoters: Promoter tac
Construction: pMMB66EH, pUC8
Marker(s):ampR
Construct size (kb): 9.064000129699707
Features: insert detection: lacZ' marker(s): ampR promoter: tac, lacUV5 replicon: oriV, oriT (IncQ) repressor gene: lacIq transcription terminator: rrnB
Applications 用途 expression vector shuttle vector
Comments 注釋 Restriction digests of the clone give the following sizes (kb): EcoRI--9.1; EcoRV--5.3, 3.8; SspI--4.9, 1.8, 1.6, 1.0; EcoRI/MluI--8.2, 0.9.
Contains sequences encoding lacIq, a tandem tac lacUV5 promoter region, and lacZalpha to allow direct screening by inactivation of alpha-complementation.
Shows a low level of expression without induction.
Media ATCC? Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 37.0°C
References 參考文獻(xiàn) Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347
Shipped freeze-dried Shipping Information Shipped: Freeze dried E. coli containing the plasmid.