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Xanthophyllomyces dendrorhous Golubev 拉丁名

(ATCC? 24202?) 菌株編號

Deposited As Phaffia rhodozyma Miller et al. 

Strain Designations 菌株別名 UCD 67-210 [ATCC 96587, CBS 5905, CCRC 21346, CCY 77-1-1, DSM 5626, IFO 10129, IGC 4172, NCYC 874, NRRL Y-10921] 

Synonym Phaffia rhodozyma Miller et al. 

Application 用途 

Produces astaxanthin Produces carotenoids 

Produces glycoproteins Increased production of carotinoids and trans-astaxanthin by addition of mevalonic acid 

Biosafety Level 生物安全等級 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 freeze-dried 

Storage Conditions 保藏方法 

Frozen 凍干粉 : -80°C or colder 

Freeze-Dried 冷凍物 : 2°C to 8°C 

Live Culture 活菌 : See Propagation Section 

Type Strain 模式菌株 yes (type strain of Phaffia rhodozyma) 

Preceptrol? no 

Comments 注釋 Taxonomy Cryopreservation Psychrophilic 

Medium 培養(yǎng)基 ATCC? Medium 200: YM agar or YM broth 

ATCC? Medium 323: Malt agar medium 

ATCC? Medium 337: Potato, dextrose, yeast agar (PDY) 

Growth Conditions 生長條件 

Temperature 培養(yǎng)溫度 : 20°C 

Atmosphere 需氧情況 : Typical aerobic 

Sequenced Data D1D2 region of the 26S ribosomal RNA gene

 ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGATGA

GCTCAAATTTGAAATCTGGCAGCCTCCGGTTGTCCGAGTTGTAAACTAGAGAAGCGTTTTCCGTGCCG

GCCTGTGTACAAGTCCCTTGGAATAGGGCGTCATAGAGGGTGAGAATCCCGTCCTTGACACAGACCAC

CGGTGCTATGTGATACGCTCTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCAAATTGGGTGGTAAAT

TCCATCTAAGGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGC

ACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCATGCGT

GCTCGGACTCAGCTGGGTTCGTCCCAGTCTATTTCCGGGTGCCGCAGGTCAGCATCAGTTTCGGGCGG

TGGAAAACGGGCGGGGGAAGGTGGCATCTCCGGATGTGTTATAGCCCCCGTTTGGATGCATCGCGTGG

GACTGAGGAACGCAGCGCGCCCTTCACGGGGTCGGTCTCCGGACACGAACGCGCTTAGGATGCTGGCATAATGGCTTTAAACGAC

Name of Depositor 寄存人 HJ Phaff 

Isolation 分離源 Fagus crenata, Kyoto, Japan 

Cross References

Nucleotide (GenBank) : AF139634 nucleotide sequence of intergenic spacer 

Nucleotide (GenBank) : AF189871 26S ribosomal RNA gene, partial sequence 

Nucleotide (GenBank) : AF139629 nucleotide sequence of internal transcribed spacer 

References 參考文獻 Johnson EA, et al. Processes for in vivo production of astaxanthin and Phaffia rhodozyma yeast of enhanced astaxanthin content. US Patent 5,356,809 dated Oct 18 1994 Golubev WI. 

Perfect state of Rhodomyces dendrorhous (Phaffia rhodozyma). Yeast 11: 101-110, 1995. PubMed: 7732720 

Miller MW, et al. Phaffia, a new yeast genus in the Deuteromycotina (Blastomycetes). Int. J. Syst. Bacteriol. 26: 286-291, 1976. . . Biotechnol. Lett. 17: 575-578, 1995. 

Hayman GT, et al. Production of carotenoids by Phaffia rhodozyma grown on media composed of corn wet-milling co-products. J. Ind. Microbiol. 14: 389-395, 1995. 

Tilser I, et al. Effect of supplementation of University of Wisconsin solution with glycoproteins from psychrophilic strains of yeast on hypothermic liver storage of rats. Cryobiology 33: 347-353, 1996. PubMed: 8689892 

Breierova E. Cryoprotection of psychrophilic yeast species- by the use of additives with croprotective media. Cryo-Lett. 15: 191-197, 1994. 

Fell JW, Blatt GM. Separation of strains of the yeasts Xanthophyllomyces dendrorhous and Phaffia rhodozyma based on rDNA IGS and ITS sequence analysis. J. Ind. Microbiol. Biotechnol. 23: 677-681, 1999. PubMed: 10455500

Fell JW, et al. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evol. Microbiol. 50: 1351-1371, 2000. PubMed: 10843082

Calo P, et al. Mevalonic acid increases trans-astaxanthin and carotenoid biosynthesis in Phaffia rhodozyma. Biotechnol Lett 17: 575-578, 1995. 

David-Palma M, Libkind D, Sampaio JP. Global distribution, diversity hot spots and niche transitions of an astaxanthin-producing eukaryotic microbe. Mol Ecol 23: 921-932, 2014. PubMed: 24372735 

Hara KY, et al. Evaluation and screening of efficient promoters to improve astaxanthin production in Xanthophyllomyces dendrorhous. Appl Microbiol Biotechnol 98: 6787-6793, 2014. PubMed: 24737060 

Loto I, et al. Enhancement of carotenoid production by disrupting the C22-sterol desaturase gene (CYP61) in Xanthophyllomyces dendrorhous. BMC Microbiol 12: 235, 2012. PubMed: 23075035 

Linde D, et al. Molecular and biochemical characterization of a beta-fructofuranosidase from Xanthophyllomyces dendrorhous. Appl Environ Microbiol 75: 1065-1073, 2009. PubMed: 19088319 

Fell JW, et al. Molecular diversity and intragenomic variability in the yeast genus Xanthophyllomyces: the origin of Phaffia rhodozyma? FEMS Yeast Res 7: 1399-1408, 2007. PubMed: 17825066 

type strain of Phaffia rhodozyma