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Kluyveromyces marxianus (EC Hansen) van der Walt 拉丁名

(ATCC? 46537?) 統(tǒng)一編號(hào) 

Deposited As Kluyveromyces fragilis (Jorgensen) van der Walt, teleomorph 

Strain Designations 菌種別名 CBS 397 [ATCC 56497, ATCC 56752, CCRC 21477, DBVPG 6164, IFO 1735, NCYC 851, NRRL Y-2415, UCD 71-58] 

Alternate State Candida kefyr (Beijerinck) van Uden et Buckley, anamorph 

Application 用途 degrades glucose D-glucose degrades whey degrades whey permeate produces alcohol 

produces ethyl alcohol ethanol produces glycerol glycerin, glycerine 

produces polyphosphates produces alcohol from whey produces ethanol from glucose 

produces glycerol from whey permeate produces extracellular polyphosphates 

Biosafety Level 生物安全等級(jí) 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 freeze-dried 

Storage Conditions 保藏方法 

Frozen 冷凍物 : -80°C or colder 

Freeze-Dried 凍干粉 : 2°C to 8°C 

Live Culture 活菌 : See Propagation Section 

Type Strain 模式菌株 yes (type strain of Kluyveromyces fragilis) 

Preceptrol? no 

Morphology On YM medium at 25°C after 4 days, colonies white to dingy white, smooth, butyrous, glistening, margin entire becoming filamentous. 

Cells hyaline, elongated ellipsoidal, smooth, 4.5-9 X 2.5-4 μm. Pseudohyphae abundant. 

Medium 培養(yǎng)基 ATCC? Medium 200: YM agar or YM broth 

Growth Conditions 生長(zhǎng)條件 

Temperature 培養(yǎng)溫度 : 24°C to 26°C 

Atmosphere 需氧情況 : Typical aerobic

Sequenced Data 18S 

ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence AAGGATCATTAAAGATTATGAATGAATAGATTACTGGGGGAATCGTCTGAACAAGGCCTGCGCTTAATTGCGCGGCCA

GTTCTTGATTCTCTGCTATCAGTTTTCTATTTCTCATCCTAAACACAATGGAGTTTTTTCTCTATGAACTACTTCCC

TGGAGAGCTCGTCTCTCCAGTGGACATAAACACAAACAATATTTTGTATTATGAAAAACTATTATACTATAAAATTTA

ATATTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAATTGCGATATGTATTGTGA

ATTGCAGATTTTCGTGAATCATCAAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCAGGGGGCATGCCTGTTTGA

GCGTCATTTCTCTCTCAAACCTTTGGGTTTGGTAGTGAGTGATACTCGTCTCGGGTTAACTTGAAAGTGGCTAGCCGT

TGCCATCTGCGTGAGCAGGGCTGCGTGTCAAGTCTATGGACTCGACTCTTGCACATCTACGTCTTAGGTTTGCGCCAA

TTCGTGGTAAGCTTGGGTCAWAGAGACTCATAGGTGTTATAAAGACTCGCTGGTGTTTGTCTCCTTGAGGCATACGGCT

TTAACCAAAACTCTCAAAGTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAA 

D1D2 region of the 28S ribosomal RNA gene 

TATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAA

ATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTTGAAGAAGGCGACTTTGTAGCTGGTCCTTGTCTAT

GTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGATCCCAGTTATTTGTAAAGTGCTTT

CGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGA

GACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAAT

TGTTGAAAGGGAAGGGCATTTGATCAGACATGGCGTTTGCTTCGGCTTTCGCTGGGCCAGCATCAGTTTTAGCGG

TTGGATAAATCCTCGGGAATGTGGCTCTGCTTCGGTAGAGTGTTATAGCCCGTGGGAATACAGCCAGCTGGGACT

GAGGATTGCGACTTTTGTCAAGGATGCTGGCGTAATGGTTAAATGCCG 

Morphology On YM medium at 25°C after 4 days, colonies white to dingy white, smooth, butyrous, glistening, margin entire becoming filamentous. 

Cells hyaline, elongated ellipsoidal, smooth, 4.5-9 X 2.5-4 μm. Pseudohyphae abundant.

Name of Depositor CBS 

Chain of Custody 來源國家 ATCC <-- CBS <-- L.C.E. Kniphorst UCD 71-58 

Isolation 分離源 Yogurt, the Netherlands 

References 參考文獻(xiàn) Van Steveninck J, et al. Isolation and characterization of polyphosphates from the yeast cell surface. Biochim. Biophys. Acta 760: 143-148, 1983. PubMed: 6615879 

Lodder J. The yeasts: a taxonomic study. 2nd ed. Amsterdam: North-Holland; 1970. Moulin G, Galzy P. Alcohol production from whey. Adv. Biotechnol. 2: 181-187, 1981. 

Rapin JD, et al. Glycerol production by yeast fermentation of whey permeate. Enzyme Microb. Technol. 16: 143-150, 1994. 

Freer SN, Detroy RW. Characterization of cellobiose fermentations to ethanol by yeasts. Biotechnol. Bioeng. 25: 541-557, 1983. type strain of Kluyveromyces fragilis