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Ogataea methanolica (Makiguchi) Kurtzman et Robnett 拉丁名

(ATCC® 58372™) 統(tǒng)一編號

Deposited As Pichia methanolica Makiguchi 

Strain Designations 菌種別名 NRRL Y-7685 [CBS 6515, CCRC 22099, IAM 12901, JCM 10240] 

Application 用途 Produces methanol oxidase 

Biosafety Level 生物安全等級 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 freeze-dried 

Storage Conditions 保藏條件

Frozen 冷凍物: -80°C or colder 

Freeze-Dried 凍干物: 2°C to 8°C 

Live Culture: See Propagation Section 

Type Strain 模式菌株 yes 

Preceptrol® no 

Genome Sequenced Strain Yes Comments Genome sequencing strain (RIKEN Center for Life Science Technologies, Japan) 

Medium 培養(yǎng)基 ATCC® Medium 28: Emmons' modification of Sabouraud's agar 

ATCC® Medium 200: YM agar or YM broth 

ATCC® Medium 1245: YEPD 

Growth Conditions 生長條件

Temperature 培養(yǎng)溫度: 24°C to 26°C 

Atmosphere 需氧情況: Typical aerobic

Name of Depositor 寄存人 NRRL Isolation Soil, Japan 

Cross References Nucleotide (GenBank) : AF141329 nucleotide sequence of MOD1 

Nucleotide (GenBank) : AF141330 nucleotide sequence of MOD2 

Nucleotide (GenBank) : BCKW00000000 Ogataea methanolica strain JCM 10240, whole genome shotgun sequencing project 

References Nakagawa T, et al. Isozymes of methanol oxidase in a methanol-utilizing yeast, Pichia methanolica IAM 12901. J. Ferment. Bioeng. 81: 498-503, 1996. 

Kato K, et al. Determination of strongly methanol assimilating yeasts. J. Gen. Appl. Microbiol. 20: 123-127, 1974. 

Nakagawa T, et al. Alcohol oxidase hybrid oligomers formed in vivo and in vitro. Yeast 15: 1223-1230, 1999. PubMed: 10487924 

Kurtzman CP, Robnett CJ. Systematics of methanol assimilating yeasts and neighboring taxa from multigene sequence analysis and the proposal of Peterozyma gen. nov., a new member of the Saccharomycetales. FEMS Yeast Res 10: 353-361, 2010. PubMed: 20522116 

Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73(4): 331-371, 1998. PubMed: 9850420 

Kurtzman CP, Robnett CJ. Relationships among genera of the Saccharomycotina (Ascomycota) from multigene phylogenetic analysis of type species. FEMS Yeast Res. 13: 23-33, 2013. PubMed: 22978764 

Ito T, et al. Molecular characterization of the PEX14 gene from the methylotrophic yeast Pichia methanolica. J. Biosci. Bioeng. 111: 624-627, 2011. PubMed: 21317032 

Nakagawa T, et al. The peroxisomal catalase gene in the methylotrophic yeast Pichia methanolica. Biosci. Biotechnol. Biochem. 74: 1733-1735, 2010. PubMed: 20699560 

Nakagawa T, et al. Molecular characterization of two genes with high similarity to the dihydroxyacetone synthase gene in the methylotrophic yeast Pichia methanolica. Biosci. Biotechnol. Biochem. 74: 1491-1493, 2010. PubMed: 20622431 

Ito T, et al. Molecular characterization of the PEX5 gene encoding peroxisomal targeting signal 1 receptor from the methylotrophic yeast Pichia methanolica. Yeast 24: 589-597, 2007. PubMed: 17506110 

Nakagawa T, et al. Regulation of two distinct alcohol oxidase promoters in the methylotrophic yeast Pichia methanolica. Yeast 23: 15-22, 2006. PubMed: 16411161 

Nakagawa T, et al. Molecular characterization of glutathione-dependent formaldehyde dehydrogenase gene FLD1 from methylotrophic yeast Pichia methanolica. Yeast 21: 445-453, 2004. PubMed: 15116345