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pPTX42pPTX42 拉丁名
(ATCC® 67046™) 統(tǒng)一編號(hào)
Designations 別名 pPTX42
GenBank Number M13223
Species Bordetella pertussis (Bergey et al.) Moreno-Lopez Depositors NIH, JM Keith, NIH Vector Construct size (kb): 14.10000038146973 Insert DNA: genomic Insert lengths(kb): 4.199999809265137 Gene product: pertussis toxin Insert Size (kb) 4.200
Media 培養(yǎng)基 ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 生物安全等級(jí) 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments 注釋 Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--10.0, 4.5; PstI--10.0, 1.5, 1.3, 1.0, 0.9; KpnI--14.5; SalI--10.0, 4.5. The insert contains the following restriction sites (nt from the EcoRI site): PstI--93, 1557, 2827, 3792; KpnI--422; SalI--929.
Contains the following coding regions (nt from the EcoRI site): S1 subunit--507 to 1313; S2 subunit--1353 to 2033; S4 subunit--2090 to 2485; S5 subunit--2497-2859; S3 subunit--2942-3625.
References 參考文獻(xiàn) Keith JM, Locht C. Pertussis toxin gene: cloning and expression of protective antigen. US Patent 4,883,761 dated Nov 28 1989
Locht C, Keith JM. Pertussis toxin gene: nucleotide sequence and genetic organization. Science 232: 1258-1264, 1986. PubMed: 3704651
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims.
Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
This material may not have been produced or characterized by ATCC.U.S. Patent 4,883,761 Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims.
Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.