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ASK [Atlantic Salmon Kidney] 拉丁名

(ATCC® CRL-2747™) 統(tǒng)一編號

Organism Salmo salar, salmon, Atlantic 

Tissue kidney 

Cell Type epithelial 

Product Format 提供形式 frozen 

Morphology epithelial 

Culture Properties adherent 

Biosafety Level 生物安全等級 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Age adult Gender female 

Applications 用途 The ASK (Atlantic Salmon Kidney) cell line was derived by B. Krossoey in 1998 from cells taken from the normal kidney of an Atlantic Salmon. 

The cell line may be used to propagate Infectious Salmon Anemia virus (ISAV) of the Atlantic Salmon. 

Storage Conditions 保藏條件 liquid nitrogen vapor phase 

Derivation Clinical Data female 

Comments 注釋 The ASK (Atlantic Salmon Kidney) cell line was derived by B. Krossoey in 1998 from cells taken from the normal kidney of an Atlantic Salmon. 

The cell line may be used to propagate Infectious Salmon Anemia virus (ISAV) of the Atlantic Salmon.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. 

To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. 

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) 

Subculturing Protocol: Subcultures are prepared by scraping. 

Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. 

Interval: Subculture every 10 to 17 days. 

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Once per week 

Cryopreservation Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO 

Storage temperature 保藏溫度 : liquid nitrogen vapor phase 

Culture Conditions 培養(yǎng)條件 

Atmosphere 需氧情況 : air, 100% 

Temperature 培養(yǎng)溫度 : 20.0°C

Name of Depositor 寄存人 J Rolland, J Winton, B Krossoey 

Deposited As Salmo salar Year of Origin 1998 References Devold M, et al. Use of RT-PCR for diagnosis of infectious salmon anaemia virus (ISAV) in carrier sea trout Salmo trutta after experimental infection. 

Dis. Aquat. Org. 40: 9-18, 2000. PubMed: 10785858