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Clostridium difficile (Hall and O'Toole) Prevot 拉丁名

(ATCC? BAA-1382?) 編號

Strain Designations 菌株別名 630 

Application 用途 Enteric Research 

Isolation 分來源 Clincal isolate, Switzerland 

Biosafety Level 安全等級 2 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 freeze-dried 

Storage Conditions 保藏方法

Frozen 冷凍物 : -80°C or colder 

Freeze-Dried 凍干物 : 2°C to 8°C 

Live Culture 需氧情況 : See Propagation Section 

Preceptrol? no 

Genotype Toxinotype: 0 Ref 

Genome Sequenced Strain Clostridium difficile 630, complete genome 

Type Strain 模式菌株 no 

Comments 注釋 Genome sequenced strain Presence of tcdA and tcdB genes confirmed by PCR. Ref Binary toxin gene cdtB not amplified by PCR Ribotype 012 Toxinotype 0

Medium 培養(yǎng)基 ATCC? Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced) 

ATCC? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood 

Growth Conditions 生長條件 

Temperature培養(yǎng)溫度 : 37°C 

Atmosphere: Anaerobic gas mixture, 80% N2 -10% CO2 -10% H2 

Genotype Toxinotype: 0 Ref 

Name of Depositor 寄存人 B Wren, H Maschler 

Isolation 分來源 Clincal isolate, Switzerland 

References Wust Jurg, et al. Investigation of an Outbreak of Antibiotic-Associated Colitis by Various Typing Methods. J. Clin. Microbiol. 16: 1096-1101, 1982. 

Sebaihia Mohammed. The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome. Nat. Genet. 38: 779-786, 2006. 

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986 

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 

Cross References Nucleotide (Genbank) : NC_009089 Clostridium difficile 630, complete genome 

Nucleotide (GenBank) : NC_008226 Clostridium difficile 630 plasmid pCD630, complete sequence. 

Nucleotide (GenBank) : AM180355 Clostridium difficile 630 complete genome.